Progesterone restores blood-brain barrier and memory after chronic cerebral hypoperfusion in male rats

Brain Behavior and Immunity Integrative Pub Date : 2025-07-01 DOI:10.1016/j.bbii.2025.100132

Maydelín Espadín , Miguel Cervantes , Angélica Coyoy-Salgado , Claudia Espinosa-Garcia , Beatriz Gómez-González , Gabriela Moralí

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Abstract

Introduction

Aging is associated with chronic cerebral hypoperfusion (CCH). These blood flow age-related alterations might be attenuated by progesterone (P4), a neurosteroid which has been proven to exert pleiotropic neuroprotective effects in brain injury models. The aim of this study was to evaluate the effects of P4 on the blood-brain barrier (BBB) and on spatial learning and memory in rats subjected to CCH.

Methods

Male Sprague-Dawley rats (12–14 month-old) were distributed in groups: CCH+vehicle; CCH+P4 and SHAM. At 7 and 14 days, the function of the BBB, the tight junction proteins and inflammatory factor levels were evaluated. At 180 days spatial learning and memory were evaluated.

Results

CCH induced BBB dysfunction, alters tight junction protein, inflammation factors and spatial learning and memory. Treatment with P4 ameliorated these alterations.

Discussion

Our results suggest that P4 plays an important role in protecting the brain from hypoperfusion in old male rats.

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黄体酮可恢复雄性大鼠慢性脑灌注不足后的血脑屏障和记忆

衰老与慢性脑灌注不足（CCH）有关。孕酮可能会减弱这些与年龄相关的血流改变（P4），孕酮是一种神经类固醇，已被证明在脑损伤模型中发挥多效神经保护作用。本研究旨在探讨P4对脑缺血大鼠血脑屏障（BBB）和空间学习记忆的影响。方法12 ~ 14月龄的Sprague-Dawley大鼠分为两组：CCH+对照；CCH+P4和SHAM。在第7天和第14天，评估血脑屏障功能、紧密连接蛋白和炎症因子水平。180 d时进行空间学习和记忆评价。结果scch诱导血脑屏障功能障碍，改变紧密连接蛋白、炎症因子和空间学习记忆。P4治疗改善了这些改变。我们的研究结果表明P4在老年雄性大鼠的脑灌注不足保护中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Brain Behavior and Immunity Integrative

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