Multifunctional AuAgPt Nanoframes for a Stimuli-Responsive Electrochemiluminescence Aptasensor.

IF 15.8 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-07-09 DOI:10.1021/acsnano.5c07691

Changxiao Song,Jingxian Li,Futing Wang,Ziyang Peng,Yan Shen,Chongyang Huang,Suping Deng,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan

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引用次数: 0

Abstract

A multifunctional electrochemiluminescence (ECL) coreaction accelerator, AuAgPt nanoframes (NFs), is described for use in an ECL aptasensor for highly sensitive aflatoxin B1 (AFB1) detection. As a signal quencher, the broad UV-vis absorption spectrum of AuAgPt nanosheets (NSs) overlaps the ECL emission spectrum of g-C3N4@Au, triggering an ECL resonance energy transfer (ECL-RET). By the adjustment of the dosage of hydrogen peroxide (H2O2), the AuAgPt NSs are transformed into AuAgPt NFs because H2O2 etches Ag in AuAgPt NSs into Ag+, which disrupts the RET process. The as-formed AuAgPt NFs act as a coreaction accelerator to enhance the ECL response of the g-C3N4@Au/K2S2O8 system. Without AFB1, the Ag-dependent DNAzyme is inactive, and a strong ECL signal is observed. After AFB1 is added, the AFB1 aptamer targets AFB1 and the DNAzyme active site is exposed. As-generated Ag+ further activates DNAzyme to cut the substrate strand (S-DNA), which causes AuAgPt NFs to detach from the electrode surface and the ECL signal to significantly decrease. Under optimal conditions, the proposed ECL aptasensor exhibits high sensitivity with a limit of detection (LOD) of 0.11 fg/mL in the range of 1 fg/mL to 1 μg/mL for AFB1 detection.

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用于刺激响应电化学发光传感器的多功能AuAgPt纳米框架。

描述了一种多功能电化学发光（ECL）协同反应促进剂，AuAgPt纳米框架（NFs），用于高灵敏度黄曲霉毒素B1 （AFB1）检测的ECL配体传感器。作为信号猝灭剂，AuAgPt纳米片（NSs）的紫外-可见吸收光谱与g-C3N4@Au的ECL发射光谱重叠，触发ECL共振能量转移（ECL- ret）。通过调整过氧化氢（H2O2）的用量，AuAgPt NSs转化为AuAgPt NFs，因为H2O2将AuAgPt NSs中的Ag蚀蚀为Ag+，从而破坏了RET过程。形成的AuAgPt NFs作为助反应加速剂，增强了g-C3N4@Au/K2S2O8体系的ECL响应。没有AFB1， ag依赖性DNAzyme是无活性的，并且观察到强烈的ECL信号。加入AFB1后，AFB1适配体靶向AFB1，暴露DNAzyme活性位点。生成的Ag+进一步激活DNAzyme切割底物链（S-DNA），导致AuAgPt NFs从电极表面分离，ECL信号显著降低。在最佳条件下，ECL感应传感器在1 fg/mL ~ 1 μg/mL范围内具有较高的灵敏度，检测限为0.11 fg/mL。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.