Low biomass bacterial transcriptomics takes shape.

IF 14 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Trends in Microbiology Pub Date : 2025-07-07 DOI:10.1016/j.tim.2025.06.015

Qinnan Yang, Eric C Martens

引用次数: 0

Abstract

Bornet et al. apply a low-input bacterial RNA-seq pipeline to transcriptionally profile small, medium, and large cell populations of the human gut symbiont Bacteroides thetaiotaomicron separated by fluorescence activated cell sorting (FACS). Their approach opens paths to measure transcriptional changes in individual cells of genetically identical bacteria that vary in morphology or other FACS-sortable features like antibody staining.

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低生物量细菌转录组学形成。

Bornet等人采用低输入的细菌RNA-seq管道，通过荧光激活细胞分选（FACS）分离人类肠道共生体拟杆菌（Bacteroides thetaiotaomicron）的小、中、大细胞群的转录谱。他们的方法为测量基因相同的细菌的单个细胞的转录变化开辟了道路，这些细菌在形态上或其他facs可分类的特征（如抗体染色）上存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Microbiology 生物-生化与分子生物学

CiteScore

25.30

自引率

0.60%

发文量

193

审稿时长

6-12 weeks

期刊介绍： Trends in Microbiology serves as a comprehensive, multidisciplinary forum for discussing various aspects of microbiology, spanning cell biology, immunology, genetics, evolution, virology, bacteriology, protozoology, and mycology. In the rapidly evolving field of microbiology, technological advancements, especially in genome sequencing, impact prokaryote biology from pathogens to extremophiles, influencing developments in drugs, vaccines, and industrial enzyme research.