Nephro-protective effect of Mucuna pruriens, Moringa oleifera, and Milk thistle extracts against APAP-induced Acute kidney injury in mice.

IF 2.3 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Kidney & blood pressure research Pub Date : 2025-07-08 DOI:10.1159/000547253

Iman Al Housseini, Hoda Dakdouk, Jamilah Borjac

{"title":"Nephro-protective effect of Mucuna pruriens, Moringa oleifera, and Milk thistle extracts against APAP-induced Acute kidney injury in mice.","authors":"Iman Al Housseini, Hoda Dakdouk, Jamilah Borjac","doi":"10.1159/000547253","DOIUrl":null,"url":null,"abstract":"Introduction: Acute kidney injury (AKI) is a rapid and often reversible decline in renal excretory function. Acetaminophen (APAP) overdose causes AKI. APAP nephrotoxicity is mostly due to the overproduction of reactive oxygen species (ROS). As no effective treatment for AKI is available, researchers are looking for natural and safe alternatives using plants. In this study, we aim to evaluate the nephroprotective effects of Moringa oleifera (Mor), Mucuna pruriens (Muc), or Milk thistle (MT) at the biochemical and molecular levels in an APAP-induced AKI mouse model.Methods: AKI was induced in mice with a single intraperitoneal (i.p.) dose of APAP (1000 mg/kg). The aqueous extracts of the three plants were given orally at 350 mg/Kg daily for 21 days pre-AKI induction. Creatinine and BUN levels were measured in serum to assess kidney function. The oxidative stress and inflammatory indicators SOD, CAT, MDA, IL-6, and TNF-α were determined using colorimetric and ELISA kits, respectively. SIRT1 protein levels were determined by western blot.Results: Pre-treatment with the three plant extracts significantly decreased the kidney biomarkers. SOD and CAT activities were enhanced with a decrease in MDA levels. IL-6 and TNF-α were also significantly lowered. A significant increase in SIRT1 expression was observed.Conclusion: Pre-treatment with Mor, Muc, and MT has a nephroprotective effect against APAP-induced kidney injury, regulating oxidative stress and inflammatory response.","PeriodicalId":17813,"journal":{"name":"Kidney & blood pressure research","volume":" ","pages":"1-24"},"PeriodicalIF":2.3000,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kidney & blood pressure research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000547253","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PERIPHERAL VASCULAR DISEASE","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Acute kidney injury (AKI) is a rapid and often reversible decline in renal excretory function. Acetaminophen (APAP) overdose causes AKI. APAP nephrotoxicity is mostly due to the overproduction of reactive oxygen species (ROS). As no effective treatment for AKI is available, researchers are looking for natural and safe alternatives using plants. In this study, we aim to evaluate the nephroprotective effects of Moringa oleifera (Mor), Mucuna pruriens (Muc), or Milk thistle (MT) at the biochemical and molecular levels in an APAP-induced AKI mouse model.

Methods: AKI was induced in mice with a single intraperitoneal (i.p.) dose of APAP (1000 mg/kg). The aqueous extracts of the three plants were given orally at 350 mg/Kg daily for 21 days pre-AKI induction. Creatinine and BUN levels were measured in serum to assess kidney function. The oxidative stress and inflammatory indicators SOD, CAT, MDA, IL-6, and TNF-α were determined using colorimetric and ELISA kits, respectively. SIRT1 protein levels were determined by western blot.

Results: Pre-treatment with the three plant extracts significantly decreased the kidney biomarkers. SOD and CAT activities were enhanced with a decrease in MDA levels. IL-6 and TNF-α were also significantly lowered. A significant increase in SIRT1 expression was observed.

Conclusion: Pre-treatment with Mor, Muc, and MT has a nephroprotective effect against APAP-induced kidney injury, regulating oxidative stress and inflammatory response.

查看原文本刊更多论文

麻瓜、辣木和水飞蓟提取物对apap诱导的小鼠急性肾损伤的保护作用。

简介：急性肾损伤（AKI）是一种肾脏排泄功能迅速且通常可逆的下降。对乙酰氨基酚（APAP）过量引起AKI。APAP肾毒性主要是由于活性氧（ROS）的过量产生。由于没有有效的治疗AKI的方法，研究人员正在寻找使用植物的天然和安全的替代品。本研究目的是在apap诱导的AKI小鼠模型中，从生化和分子水平评价辣木（Moringa oleifera, Mor）、麻豆（Mucuna pruriens, Muc）和水飞蓟（Milk thisle， MT）的肾保护作用。方法：单次腹腔注射APAP （1000 mg/kg）诱导小鼠AKI。三种植物的水提物以350 mg/Kg / d / d的剂量口服，连续21 d进行aki诱导。测定血清肌酐和BUN水平以评估肾功能。分别采用比色法和酶联免疫吸附法检测各组氧化应激和炎症指标SOD、CAT、MDA、IL-6和TNF-α。western blot检测SIRT1蛋白水平。结果：三种植物提取物预处理显著降低肾脏生物标志物。SOD和CAT活性随着MDA水平的降低而增强。IL-6和TNF-α也显著降低。观察到SIRT1表达显著增加。结论：Mor、Muc和MT预处理对apap诱导的肾损伤具有肾保护作用，可调节氧化应激和炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Kidney & blood pressure research 医学-泌尿学与肾脏学

CiteScore

4.80

自引率

3.60%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal comprises both clinical and basic studies at the interface of nephrology, hypertension and cardiovascular research. The topics to be covered include the structural organization and biochemistry of the normal and diseased kidney, the molecular biology of transporters, the physiology and pathophysiology of glomerular filtration and tubular transport, endothelial and vascular smooth muscle cell function and blood pressure control, as well as water, electrolyte and mineral metabolism. Also discussed are the (patho)physiology and (patho) biochemistry of renal hormones, the molecular biology, genetics and clinical course of renal disease and hypertension, the renal elimination, action and clinical use of drugs, as well as dialysis and transplantation. Featuring peer-reviewed original papers, editorials translating basic science into patient-oriented research and disease, in depth reviews, and regular special topic sections, ''Kidney & Blood Pressure Research'' is an important source of information for researchers in nephrology and cardiovascular medicine.