Correlative light and electron microscopy analysis for neurobiological applications.

Abstract

In this article, we will introduce the significance of correlative light and electron microscopy analyses, an integrated morphological analysis method that directly combines laser and electron microscopes. This protocol comprehensively instructs the appropriate methods for the visualization of genetically labeled neurons in mice using digitalized electron microscopes. We wish to consider with the readers the importance and functional potential of electron microscopic analysis for medical and biological applications, especially for neurobiological applications.