The c-di-GMP Metabolic Gene pdeN Interacts with LacY and ManZ to Modulate Biofilm Formation in Avian Pathogenic Escherichia coli.

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Zhihao Wang, Xiaolong Lv, Lanfang Kong, Saqib Nawaz, Chuanyan Che, Zhaoguo Chen, Huifang Yin, Cuiqin Huang, Yinli Bao, Wei Jiang, Xiangan Han
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Abstract

Bis-(3'-5')-cyclic diguanylic acid (c-di-GMP), a ubiquitous secondary messenger, affects multiple biological characteristics, including biofilm formation in avian pathogenic Escherichia coli (APEC). C-di-GMP is synthesized by diguanylate cyclase harboring a GGDEF domain and degraded by phosphodiesterase harboring either an EAL or an HD-GYP domain. However, the roles of PdeN, encoding a CSS-EAL domain, are uncharacterized. In this study, it is demonstrated that lacking pdeN significantly promotes biofilm formation and reduces the motility of the clinically isolated APEC O2 serotype strain DE17. In addition, macrocolony morphotypes showed that the ΔpdeN strain exhibits increasing production of curli fibers and cellulose, which is consistent with the results of RNA-seq and qPCR. Further exploration shows that lactose permease LacY and mannose permease subunit ManZ interact with PdeN. Infection experiments show that lacking pdeN significantly reduced the release of LDH in HD-11 cells and adhesion capacity to DF-1 cells. In conclusion, c-di-GMP metabolic gene pdeN involves biofilm formation and pathogenicity of APEC. Besides, it interacts with LacY and ManZ. Those results provide a basis for the prevention and control of APEC from the perspective of biofilm and carbohydrate metabolism.

c-二gmp代谢基因pdeN与LacY和ManZ相互作用调控禽致病性大肠杆菌生物膜形成
双-(3'-5')-环双胍酸(c-di-GMP)是一种普遍存在的次级信使,影响多种生物学特性,包括禽致病性大肠杆菌(APEC)生物膜的形成。c -二gmp由含有GGDEF结构域的二胍酸环化酶合成,并由含有EAL或HD-GYP结构域的磷酸二酯酶降解。然而,编码CSS-EAL域的PdeN的作用是未知的。本研究表明,缺乏pdeN可显著促进临床分离的APEC O2血清型菌株DE17的生物膜形成并降低其运动性。此外,大菌落形态显示ΔpdeN菌株卷曲纤维和纤维素的产量增加,这与RNA-seq和qPCR的结果一致。进一步的研究表明,乳糖渗透酶LacY和甘露糖渗透酶亚基ManZ与PdeN相互作用。感染实验表明,缺乏pdeN可显著降低HD-11细胞中LDH的释放和对DF-1细胞的粘附能力。综上所述,c-二gmp代谢基因pdeN参与了APEC的生物膜形成和致病性。此外,它还与LacY和ManZ互动。这些结果为从生物膜和碳水化合物代谢的角度防治APEC提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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