{"title":"Circulating Levels of miR-155 and CTBP1-AS2 as a Promising Biomarker for Early Detection of Diabetic Nephropathy.","authors":"Arezoo Rahimi, Shekoofeh Nikooei, Khatere Roozbehi, Davood Semirani-Nezhad, Rozina Abbasi Larki, Arash Arya, Danial Gholami, Behnam Alipoor","doi":"10.1007/s10528-025-11176-2","DOIUrl":null,"url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is one of the most frequent complications of diabetes mellitus. Since the early diagnosis of DN is crucial to prevent the progression of the disease towards renal failure, many efforts have been made in recent years to introduce new diagnostic biomarkers. Recent studies suggest that non-coding RNAs could act as a novel diagnostic biomarker for the early detection and prediction of DN progress. Accordingly, in the current study we investigated the expression levels of miR-155 and CTBP1-AS2 in type 2 diabetes (T2D), DN patients and control subjects, and evaluated their diagnostic potential for DN. A total of 189 age and sex-matched subjects including 65 T2D patients with normo-albuminuria, 61 DN patients who had a history of albuminuria, and 63 control subjects were included in this case-control study. The expression levels of miR-155 and CTBP1-AS2 were determined using QRT-PCR. The results revealed that the expression level of miR-155 was significantly reduced in T2D patients. In addition, miR-155 level was significantly higher in DN patients with macroalbuminuria compared to DN patients with microalbuminuria and T2D patients with normo-albuminuria. The expression level of CTBP1-AS2 in T2D without proteinuria was higher than DN subjects with macroalbuminuria. The results also showed that there was a significant positive correlation between the miR-155 level with DBP, TG, TC, SCr and, BUN levels and a negative correlation with HDL-C and eGFR values. Deregulation levels of miR-155 and CTBP1-AS2 may represent useful novel diagnostic biomarkers for DN.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10528-025-11176-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Diabetic nephropathy (DN) is one of the most frequent complications of diabetes mellitus. Since the early diagnosis of DN is crucial to prevent the progression of the disease towards renal failure, many efforts have been made in recent years to introduce new diagnostic biomarkers. Recent studies suggest that non-coding RNAs could act as a novel diagnostic biomarker for the early detection and prediction of DN progress. Accordingly, in the current study we investigated the expression levels of miR-155 and CTBP1-AS2 in type 2 diabetes (T2D), DN patients and control subjects, and evaluated their diagnostic potential for DN. A total of 189 age and sex-matched subjects including 65 T2D patients with normo-albuminuria, 61 DN patients who had a history of albuminuria, and 63 control subjects were included in this case-control study. The expression levels of miR-155 and CTBP1-AS2 were determined using QRT-PCR. The results revealed that the expression level of miR-155 was significantly reduced in T2D patients. In addition, miR-155 level was significantly higher in DN patients with macroalbuminuria compared to DN patients with microalbuminuria and T2D patients with normo-albuminuria. The expression level of CTBP1-AS2 in T2D without proteinuria was higher than DN subjects with macroalbuminuria. The results also showed that there was a significant positive correlation between the miR-155 level with DBP, TG, TC, SCr and, BUN levels and a negative correlation with HDL-C and eGFR values. Deregulation levels of miR-155 and CTBP1-AS2 may represent useful novel diagnostic biomarkers for DN.
期刊介绍：
Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses.
Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication.
Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses.
Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods.
Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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