Titanium surface modified with nanostructure mimicking the surface of natural cementum up-regulated epithelial expression of integrin β4 and enhanced the attachment of the transmucosal gingiva.
Zhaoming Deng, Lerong Yu, Yi Sun, Yishen Kuang, Ziyao Zhou, Mengyu Chen, Xiangwei Li
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引用次数: 0
Abstract
Objective: Biomimetic materials are increasingly used for tissue repair and regenerative medicine. However, in implant dentistry, especially in field focusing on the improvement of gingival adhesion and sealing, little has been reported so far. To be specific, it remains unclear whether biomimetic topography of the titanium surface mimicking natural cementum would have an enhancing effect on the gingival adhesion.
Methods: In the present study, we characterized the topography of the deproteinized cementum and fabricated nanotubes on the titanium surface with features simulating natural cementum surface via electrochemical anodization. Then we cultured the human gingival fibroblasts (HGFs) on the modified surface to evaluate the biocompatibility and the effect on morphological changes of the cells. Besides, in vivo assay was performed to assess whether the modified surface of mini-implants would improve the gingival adhesion.
Results: Histological evaluation confirmed the interaction between gingival collagen fibers and cementum, and the topography of the cementum consisted of a large number of pores and holes in micro- and nanoscale. After the anodization, densely or loosely distributed nanotubes mimicking the topography of cementum were fabricated on the surface of titanium, with diameter of approximately 60, 100 and 120 nm under 30, 40 and 50 voltages respectively (TNT-30/40/50). Compared to the polish surface and TNT-50 surface, densely distributed nanotubes (TNT-30/40) increased cell area and aspect ratio of human gingival fibroblasts (p < 0.05). Besides, mini-implants of TNT-30 and TNT-40 enhanced the expression of integrin β4 in transmucosal epithelium.
Significance: Densely distributed nanotubes topography with features mimicking the surface of natural cementum was favorable for gingival fibroblasts and epithelial cells to achieve tight adhesion on the surface of the materials. Biomimetically modified titanium surface contributed to enhanced gingival sealing and integration of gingival soft tissue around the dental implant.
期刊介绍：
Dental Materials publishes original research, review articles, and short communications.
Academy of Dental Materials members click here to register for free access to Dental Materials online.
The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology.
Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.
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