Exploring Genetic Diversity in a Core Collection of Aegilops tauschii Coss. Populations Using iPBS and SCoT Markers.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2025-07-09 DOI:10.1007/s10528-025-11178-0

Hoda Moradkhani, Alireza Pour-Aboughadareh, Bita Jamshidi, Omid Jadidi, Ali Ashraf Mehrabi, Aras Türkoğlu, Jan Bocianowski

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引用次数: 0

Abstract

Evaluation of population structure and genetic diversity is one of the primary steps in any plant breeding program. Wheat (Triticum aestivum L.) is a key staple cereal crop that plays an important role in global food security. The development of improved wheat varieties and the broadening of their genetic base depends on identifying novel allelic variations within germplasm resources. Hence, this study aimed to analyze the genetic diversity and population structure of 111 selected Aegilops tauschii Coss. accessions-the donor of the D-genome in bread wheat-collected from different countries, using Start Codon Targeted (SCoT) and inter-Primer Binding Site (iPBS) molecular markers. Ten selected primers from the SCoT and iPBS marker systems amplified a total of 108 and 147 fragments, respectively. Key informativeness parameters, including the number of polymorphic fragments (NPF), polymorphic information content (PIC), marker index (MI), and resolving power (R_p), were estimated as 10.80/14.70, 0.38/0.41, 4.12/6.13, and 12.90/16.31 for SCoT and iPBS primers, respectively. Analysis of molecular variance (AMOVA) indicated that genetic variation within regions was greater than variation among them. Among the studied populations, those from Iran and Afghanistan exhibited the highest levels of genetic diversity. Multivariate analyses showed that grouping patterns largely corresponded to the geographical origins of the accessions. These results were further validated by population structure analysis, which confirmed distinct genetic classifications based on SCoT, iPBS, and combined marker data. In conclusion, our results highlight that the combining SCoT and iPBS markers can be a robust approach for genotyping and assessing genetic diversity in plant germplasms. These insights are valuable for wheat breeding programs, aiding in the identification and utilization of diverse germplasm and managing it for crop improvement.

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黄花盾核心群遗传多样性的研究。使用iPBS和SCoT标记的人群。

群体结构和遗传多样性的评估是任何植物育种计划的基本步骤之一。小麦（Triticum aestivum L.）是一种重要的主要谷类作物，在全球粮食安全中发挥着重要作用。小麦改良品种的开发及其遗传基础的扩大取决于在种质资源中发现新的等位基因变异。因此，本研究旨在分析111份选择的黄颡鱼（Aegilops tauschii）遗传多样性和群体结构。使用起始密码子靶向（SCoT）和引物结合位点（iPBS）分子标记从不同国家收集了面包小麦d基因组的供体。从SCoT和iPBS标记系统中选择的10个引物分别扩增了108个和147个片段。SCoT和iPBS引物的关键信息性参数，包括多态性片段数（NPF）、多态性信息含量（PIC）、标记指数（MI）和分辨能力（Rp）分别为10.80/14.70、0.38/0.41、4.12/6.13和12.90/16.31。分子变异分析（AMOVA）表明，区域内的遗传变异大于区域间的遗传变异。在研究人群中，来自伊朗和阿富汗的人群表现出最高水平的遗传多样性。多变量分析表明，分组模式与资源的地理来源基本一致。群体结构分析进一步验证了这些结果，基于SCoT、iPBS和联合标记数据证实了不同的遗传分类。总之，我们的研究结果表明，结合SCoT和iPBS标记可以作为植物种质资源基因分型和遗传多样性评估的可靠方法。这些见解对小麦育种计划很有价值，有助于鉴定和利用各种种质资源，并对其进行管理以促进作物改良。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.