Critical Method Parameters to Evaluate When Developing a Method Using a Laser Diode Thermal Desorption (LDTD) Coupled to a Mass Spectrometer

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-07-09 DOI:10.1002/rcm.10104

Jonathan Rochon, Serge Auger, Eshwar Jagerdeo

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Abstract

Rationale

This paper presents an in-depth evaluation of the laser diode thermal desorption (LDTD) device interfaced to a mass spectrometer and identifies the critical method parameters to evaluate when developing a new procedure.

Methods

Samples were solvent extracted and spotted in a 96-well plate. In the case of biological fluids, hydrolysis followed by solid phase extraction is required. The solvent in the 96-well plate is evaporated, followed by mass spectrometric analysis (MS) with atmospheric pressure chemical ionization (APCI). Where applicable, the instrument is operated in a data-dependent mode, with a full-scan mass spectrum followed by MS/MS spectra with a total runtime of 18 s per well.

Results

Five key experiments (sample dilution, gas flow, laser ramp, ion saturation/ion suppression, and signal enhancement) were evaluated as critical parameters that could potentially be encountered in method development, and practical solutions are proposed.

Conclusions

Interfacing LDTD with a mass spectrometer allows for rapid screening for many analytes in a wide range of samples, with either minimal or complex sample preparation. Addressing all critical method parameters can aid in creating a sensitive and robust procedure.

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使用激光二极管热解吸（LDTD）耦合质谱仪开发方法时需要评估的关键方法参数

本文对与质谱联用的激光二极管热脱附（LDTD）装置进行了深入的评估，并确定了在开发新程序时需要评估的关键方法参数。方法采用溶剂提取法，在96孔板上进行斑点检测。在生物流体的情况下，需要水解，然后是固相萃取。蒸发96孔板中的溶剂，采用常压化学电离（APCI）进行质谱分析（MS）。在适用的情况下，仪器以数据依赖模式运行，具有全扫描质谱，然后是MS/MS谱，每口井的总运行时间为18秒。结果5个关键实验（样品稀释、气体流动、激光斜坡、离子饱和/离子抑制和信号增强）被评估为方法开发中可能遇到的关键参数，并提出了可行的解决方案。将LDTD与质谱仪相结合，可以快速筛选大量样品中的许多分析物，只需少量或复杂的样品制备。处理所有关键的方法参数可以帮助创建一个敏感和健壮的过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.