Overexpression of the ABA-responsive transcription factor PtrMYBH alters shoot apical meristem, xylem, root, and leaf development in Populus

IF 6.2 1区生物学 Q1 PLANT SCIENCES

The Plant Journal Pub Date : 2025-07-09 DOI:10.1111/tpj.70337

Ziqi Liu, Xiaomeng Fan, Xuqing Liu, Zeyu Zhang, Xiangrong Li, Yingjie Yan, Shengcai Xiang, Xiaoqi Zhang, Tingting Lu, Siyu Lu, Dengcan Liu, Tian Hua, Mengna Zhao, Jun Zhang, Hangxiao Zhang, Lianfeng Gu

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引用次数: 0

Abstract

Abscisic acid (ABA) mediates stress responses and growth regulation in plants, but the roles of ABA-responsive transcription factors (TFs) in Populus development remain poorly characterized. Here, we identified an ABA-upregulated TF and investigated its function through overexpression in transgenic poplar. The PtrMYBH is upregulated during ABA treatment, and its overexpression in transgenic poplar leads to leaf malformations, including reduced size and curling, with severity correlating with PtrMYBH-overexpression levels in three independent transgenic lines. PtrMYBH regulates stomatal growth and development, resulting in decreased stomatal length and aperture, along with distinct leaf structural abnormalities. Additionally, PtrMYBH affects root development, with overexpressing lines showing an increase in adventitious root number but shorter lengths, alongside morphological changes in the root elongation zone. Furthermore, morphological changes are observed in the shoot apical meristem (SAM) and stem-differentiating xylem (SDX) of PtrMYBH-overexpressing poplar. RNA-seq analyses reveal PtrMYBH's influence on the expression of genes related to cellular proliferation in the SAM and developmental pathways in the SDX. Finally, in PtrMYBH-overexpressing lines, ABA treatment results in leaf tip damage, earlier leaf drop, and stunted growth, highlighting its critical role in the ABA response. These findings lay a foundation for further exploration of TFs like PtrMYBH to regulate growth in Populus species.

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aba应答转录因子PtrMYBH的过度表达改变了杨树茎尖分生组织、木质部、根和叶片的发育

脱落酸（ABA）介导植物的胁迫响应和生长调节，但ABA应答转录因子（TFs）在杨树发育中的作用尚不清楚。本研究鉴定了aba上调的TF，并通过在转基因杨树中的过表达研究了其功能。PtrMYBH在ABA处理过程中表达上调，其在转基因杨树中的过表达导致叶片畸形，包括尺寸减小和卷曲，其严重程度与三个独立转基因品系的PtrMYBH过表达水平相关。PtrMYBH调控气孔生长发育，导致气孔长度和孔径减小，叶片结构明显异常。此外，PtrMYBH影响根系发育，过表达系不定根数量增加，但长度缩短，同时根系伸长区形态发生变化。此外，ptrmybh过表达的杨树的茎尖分生组织（SAM）和茎分化木质部（SDX）也发生了形态学变化。RNA-seq分析揭示了PtrMYBH对SAM和SDX发育途径中细胞增殖相关基因表达的影响。最后，在ptrmybh过表达品系中，ABA处理导致叶尖损伤、叶早落和生长发育迟缓，突出了其在ABA响应中的关键作用。这些发现为进一步探索PtrMYBH等tf调控杨树物种生长奠定了基础。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.