Dual-organelle targeting regulation of pyrene probes for organelles imaging in living cells

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-07-09 DOI:10.1016/j.dyepig.2025.113020

Ying Cao , Yunjia Xu , Linshuai Yang , Qi Ai , Baoxiang Gao

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Abstract

The technique of dual-organelle labeling with a single fluorescent probe offers a powerful approach for studying inter-organellar interactions, despite its inherent technical challenges. In this work, we designed and synthesized two charge-transfer probes, PN-1 and PN-2, based on a pyrene scaffold. Both probes feature an azetidine moiety as the electron donor, paired with pyridine (PN-1) and pyridin-2(1H)-one (PN-2) as distinct electron acceptors. Notably, due to the divergent electron-withdrawing capacities and hydrophilic-lipophilic balances of their acceptor units, PN-1 enables simultaneous targeting of the endoplasmic reticulum (ER) and lipid droplets (LDs), whereas PN-2 labels the ER and Golgi apparatus in live cells. Using PN-1, dynamic ER-LD interactions were visualized under oleic acid exposure and starvation stress. Furthermore, PN-2 was shown to enable real-time monitoring of ER polarity fluctuations in response to diverse stressors. These environmentally sensitive fluorescent probes establish an ideal design platform for studying inter-organellar crosstalk, holding significant promise for advancing research in cellular biology and related fields.

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双细胞器靶向调控的芘探针在活细胞细胞器成像中的应用

单荧光探针双细胞器标记技术为研究细胞器间相互作用提供了一种强有力的方法，尽管其固有的技术挑战。在这项工作中，我们设计并合成了两个基于芘支架的电荷转移探针PN-1和PN-2。两种探针都以氮杂啶片段作为电子给体，与吡啶（PN-1）和吡啶-2(1H)- 1 （PN-2）配对作为不同的电子受体。值得注意的是，由于它们的受体单位具有不同的吸电子能力和亲水-亲脂平衡，PN-1可以同时靶向内质网（ER）和脂滴（ld），而PN-2则标记活细胞中的内质网和高尔基体。利用PN-1，观察了油酸暴露和饥饿胁迫下ER-LD的动态相互作用。此外，PN-2被证明能够实时监测响应不同应激源的ER极性波动。这些环境敏感型荧光探针为研究胞间串扰提供了理想的设计平台，对推进细胞生物学及相关领域的研究具有重要的前景。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.