Generation of three human erythroblast-derived iPSC lines (UBTi002-A, UBTi002-B, and UBTi002-C)

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-05 DOI:10.1016/j.scr.2025.103767

Martina Auer, Hannah Sophie Klampfl, Peter Schlenke, Isabel Dorn

引用次数: 0

Abstract

We reprogrammed human hematopoietic stem/progenitor cell (HSPC)-derived erythroblasts obtained from a healthy male donor. CD34+ HSPCs were differentiated ex-vivo into erythroid precursor cells and were then nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-MYC, and TP53-shRNA. The resulting iPSC lines displayed a normal karyotype. Pluripotency was confirmed through the expression of markers for undifferentiated human pluripotent stem cells (hPSC) and in vitro differentiation into tissues representing endoderm, mesoderm, and ectoderm. The iPSC lines UBTi002-A, UBTi002-B, and UBTi002-C are expected to serve as valuable tools for advancing research in human hematopoiesis and erythropoiesis.

查看原文本刊更多论文

三种人红母细胞衍生的iPSC系（UBTi002-A、UBTi002-B和UBTi002-C）的生成

我们重新编程了从健康男性供体获得的人造血干细胞/祖细胞（HSPC）衍生的红母细胞。CD34+ HSPCs在体外分化为红系前体细胞，然后用表达SOX2、OCT3/4、KLF4、LIN28、L-MYC和TP53-shRNA的4种外生质粒进行核转染。结果显示iPSC系的核型正常。多能性通过表达未分化的人多能干细胞（hPSC）标记物和体外分化为代表内胚层、中胚层和外胚层的组织来证实。iPSC细胞系UBTi002-A、UBTi002-B和UBTi002-C有望成为推进人类造血和红细胞生成研究的宝贵工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.