Generation of chicken induced pluripotent stem cells (iPSCs) from somatic cells using multi-lentiviral transduction and OAC2

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-07-07 DOI:10.1016/j.theriogenology.2025.117567

Guangzheng Liu , Xilin Zhu , Zeyu Li , Liqian Zhu , Guohong Chen , Yingjie Niu , Hongyan Sun , Qisheng Zuo , Jiuzhou Song , Wei Han , Wanhong Wei , Bichun Li , Kai Jin

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引用次数: 0

Abstract

The chicken iPSCs have been successfully established and exhibit significant potential for the recovery and conservation of endangered avian species. However, the efficiency of induction remains quite limited. To enhance the induction system, a six-factor reprogramming approach incorporating OCT4, SOX2, NANOG, LIN28, KLF4, and C-MYC (OSNLKM) was employed via lentiviral infection, in combination with the small-molecule compound OAC2 and mouse embryonic fibroblast (MEF) feeder layers. The results demonstrated that the six-factor system significantly accelerated the reprogramming process and improved induction efficiency compared to the conventional four-factor system (OCT4, SOX2, NANOG, and LIN28; OSNL). Additionally, the MEF feeder layers were found to be essential for the successful reprogramming of chicken somatic cells. The inclusion of Oct4-activating compound 2 (OAC2) further optimized the reprogramming conditions, enabling the successful induction of iPSCs from chicken embryonic fibroblasts (CEFs) and Sertoli cells. Collectively, this study establishes an optimized reprogramming system for chicken somatic cells, enhancing iPSCs induction efficiency and expanding the potential applications of iPSCs technology in avian conservation and genetic modification.

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利用多慢病毒转导和OAC2诱导体细胞生成鸡诱导多能干细胞（iPSCs）

鸡iPSCs已成功建立，对濒危鸟类的恢复和保护具有重要的潜力。然而，感应的效率仍然相当有限。为了增强诱导系统，采用六因子重编程方法，包括OCT4、SOX2、NANOG、LIN28、KLF4和C-MYC (OSNLKM)，通过慢病毒感染，结合小分子化合物OAC2和小鼠胚胎成纤维细胞（MEF）喂养层。结果表明，与传统的四因子体系（OCT4、SOX2、NANOG和LIN28）相比，六因子体系显著加快了重编程过程，提高了诱导效率；OSNL)。此外，MEF饲喂层被发现是成功重编程鸡体细胞的关键。oct4激活化合物2 （OAC2）的加入进一步优化了重编程条件，使鸡胚成纤维细胞（CEFs）和Sertoli细胞成功诱导iPSCs。总之，本研究建立了一个优化的鸡体细胞重编程系统，提高了iPSCs的诱导效率，扩大了iPSCs技术在禽类保护和基因改造中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.