Modulation of optical heating during hyperthermia for the separation of reversible and irreversible changes in biological tissues

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology Pub Date : 2025-07-01 DOI:10.1016/j.jtherbio.2025.104192

Kirill Sovin , Nikita Kovalenko , Oleg Ryabushkin

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引用次数: 0

Abstract

Preoperative planning of medical attention is crucial in minimally-invasive laser, microwave and radiofrequency treatments. For this purpose, it is important to determine accurately the parameters of biological tissues that describe its changes that occur during hyperthermia procedures.

A method providing spatially uniform heating was developed during which changes in the modulus of the electrical admittance of the sample at a frequency of 10 kHz corresponding to the β-dispersion region which is most sensitive to degradation processes were recorded. Highly sensitive fiber Bragg gratings were used for temperature measurements.

Amplitude modulation of optical radiation power (20 W at 970 nm) enabled the separation of reversible and irreversible changes occurring during the heating process. Reversible changes are related to the temperature dependence of the ionic conductivity, and irreversible ones are related to cell lysis and protein coagulation.

Experimental data were obtained for chicken liver samples. The data were analyzed using the Arrhenius formalism describing the irreversible degradation process of biological tissue. The values of the critical temperature

T_{c r} = 101 \pm 2 ° С

and the logarithm of the frequency factor

\ln (A) = (48.5 \pm 3.8) \ln (s^{- 1})

were obtained.

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在生物组织中分离可逆和不可逆变化的热疗过程中光学加热的调制

在微创激光、微波和射频治疗中，术前医疗护理计划是至关重要的。为此，重要的是准确地确定生物组织的参数，以描述其在热疗过程中发生的变化。开发了一种提供空间均匀加热的方法，在此过程中记录了样品的电导纳模量在10 kHz频率下的变化，对应于对降解过程最敏感的β-色散区域。高灵敏度光纤布拉格光栅用于温度测量。光辐射功率的调幅（970 nm处20 W）使加热过程中发生的可逆和不可逆变化得以分离。可逆变化与离子电导率的温度依赖性有关，不可逆变化与细胞裂解和蛋白质凝固有关。获得了鸡肝样品的实验数据。用描述生物组织不可逆降解过程的阿伦尼乌斯公式对数据进行分析。得到了临界温度Tcr=101±2°С和频率因子ln(A)的对数=(48.5±3.8)ln （s−1）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of thermal biology 生物-动物学

CiteScore

5.30

自引率

7.40%

发文量

196

审稿时长

14.5 weeks

期刊介绍： The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles