The routine use of a digital tool for the tumor cell fraction quantification in molecular pathology: an international validation of QuANTUM.

IF 4.4 Q1 PATHOLOGY

PATHOLOGICA Pub Date : 2025-06-01 DOI:10.32074/1591-951X-1100

Vincenzo L'Imperio, Giulia Capitoli, Giorgio Cazzaniga, Mauro Mannino, Francesca Bono, Davide Seminati, Catarina Eloy, Joao Pinto, Elena Guerini Rocco, Matteo Fassan, Pasquale Pisapia, Francesco Pepe, Lara Pijuan, Jordi Temprana-Salvador, Antonio Polonia, Syed Ali Khurram, Emanuela Bonoldi, Alessandro Marando, Giuseppe Perrone, Stefania Galimberti, Giancarlo Troncone, Umberto Malapelle, Fabio Pagni

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引用次数: 0

Abstract

Objective: The absolute and relative quantification of tumor cell fraction (TCF) in tissue samples for molecular pathology testing is time-consuming and poorly reproducible.

Methods: Here we report the results of an international survey on non-small cell lung cancer (NSCLC), validating the Qupath Analysis of Nuclei from Tumor to Uniform Molecular tests (QuANTUM) automated computational pipeline for TCF quantification.

Results: The TCF obtained with QuANTUM is reliable, as demonstrated by the comparison with the manual counting of cells (ground truth, GT) in cell blocks, small biopsies and surgical specimens (overall correlation of 0.89). The visual evaluation of QuANTUM-processed images increased the pathologists' agreement with GT and QuANTUM of +0.16, +0.21, +0.09 and +0.17, +0.29, +0.21 across the three sample types, respectively. An overall increase in cases classified as containing ≥100 tumor cells for all sample types was noted after QuANTUM (from 75 cases, 63% to 96 cases, 80% among cell blocks, p = 0.003).

Conclusions: QuANTUM is an easy-to-use and reliable tool for the TCF assessment and its employment significantly modifies the visual estimation by pathologists, improving the assessment of NSCLC cases for molecular analysis.

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分子病理学中肿瘤细胞分数定量的数字工具的常规使用：量子的国际验证。

目的：用于分子病理学检测的组织样本中肿瘤细胞分数（TCF）的绝对定量和相对定量耗时且重复性差。方法：本文报告了一项关于非小细胞肺癌（NSCLC）的国际调查结果，验证了从肿瘤细胞核到均匀分子测试（QuANTUM）自动计算管道的四路径分析，用于TCF定量。结果：与细胞块、小活检和手术标本的人工细胞计数（ground truth， GT）（总相关性为0.89）相比，QuANTUM获得的TCF是可靠的。在三种样本类型中，QuANTUM处理图像的视觉评价增加了病理学家与GT和QuANTUM的一致性，分别为+0.16,+0.21,+0.09和+0.17,+0.29,+0.21。在QuANTUM之后，所有样本类型中被分类为含有≥100个肿瘤细胞的病例总体增加（从75例，63%到96例，80%的细胞块，p = 0.003）。结论：QuANTUM是一种易于使用和可靠的TCF评估工具，它的使用显著改变了病理学家的视觉估计，提高了对NSCLC病例的分子分析评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PATHOLOGICA PATHOLOGY-

CiteScore

5.90

自引率

5.70%

发文量

108