QTL combinations associated with field partial resistance to Aphanomyces root rot in pea near-isogenic lines.

IF 4.2 1区农林科学 Q1 AGRONOMY

Theoretical and Applied Genetics Pub Date : 2025-07-08 DOI:10.1007/s00122-025-04961-5

C Lavaud, J-P Riviere, P Vetel, G Aubert, P Marget, G Roullet, J F Herbommez, P Declerck, M-L Pilet-Nayel

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引用次数: 0

Abstract

Aphanomyces root rot, caused by Aphanomyces euteiches, is one of the most-important diseases of pea (Pisum sativum L.) worldwide. The development of resistant varieties is a major objective to manage the disease. Consistent quantitative trait loci (QTL) controlling partial resistance were discovered from linkage mapping and genome-wide association studies. This study aimed to validate the resistance QTL effects and identify effective QTL combinations under contaminated field conditions, by exploiting near-isogenic lines (NILs) carrying resistance alleles at individual or combined consistent QTL in different genetic backgrounds. A total of 157 NILs previously created were fingerprinted using 10,494 SNP markers from the GenoPea Infinium® BeadChip, which made it possible to confirm the QTL introgression sizes in the NILs. All NILs were phenotyped for resistance in field-contaminated nurseries over eight environments in 2 years at six locations in France. NILs carrying resistance alleles from PI180693 or 90-2131 at the major-effect QTL Ae-Ps7.6, individually or in combination with minor-effect QTL (Ae-Ps4.1 or Ae-Ps5.1), showed increased levels of partial resistance in at least three environments and three genetic backgrounds. At other QTL combinations (Ae-Ps1.2 or Ae-Ps7.6 + Ae-Ps2.2 ± Ae-Ps3.1), alleles from PI180693 or 552 also showed consistent and novel effects on partial resistance in some NIL genetic backgrounds. At these QTL combinations, the PI180693 resistance alleles also contributed to late flowering. This study provides tools and information for the choice of resistance QTL to combine in breeding, to increase partial resistance to A. euteiches in pea varieties.

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豌豆近等基因系田间部分抗根腐病的QTL组合

豌豆根腐病是豌豆（Pisum sativum L.）最重要的病害之一，由欧洲豌豆根腐病（Aphanomyces euteiches）引起。培育抗病品种是控制该病的主要目标。通过连锁定位和全基因组关联研究，发现了控制部分抗性的一致数量性状位点。本研究旨在利用不同遗传背景下携带抗性等位基因的近等基因系（NILs），在单个或组合一致的QTL上，验证抗性QTL效应，并在污染条件下鉴定有效的QTL组合。使用来自GenoPea Infinium®BeadChip的10494个SNP标记，对先前创建的157个NILs进行指纹识别，从而可以确认NILs中的QTL渗入大小。所有NILs在法国6个地点2年8个环境的田间污染苗圃中进行抗性表型分析。在主效QTL Ae-Ps7.6上携带来自PI180693或90-2131的抗性等位基因的NILs，单独或与次效QTL Ae-Ps4.1或Ae-Ps5.1组合，在至少三种环境和三种遗传背景下显示出部分抗性水平的增加。在其他QTL组合（Ae-Ps1.2或Ae-Ps7.6 + Ae-Ps2.2±Ae-Ps3.1）中，来自PI180693或552的等位基因在一些NIL遗传背景下也对部分抗性表现出一致的新影响。在这些QTL组合中，PI180693抗性等位基因对晚花也有贡献。本研究为选择抗病QTL组合进行育种，提高豌豆品种对黄粉豌豆的部分抗性提供了工具和信息。

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来源期刊

Theoretical and Applied Genetics 生物-农艺学

CiteScore

9.60

自引率

7.40%

发文量

241

审稿时长

2.3 months

期刊介绍： Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.