Nucleotides enriched under heat stress recruit beneficial rhizomicrobes to protect plants from heat and root-rot stresses.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY
Haijiao Liu, Yingwei Su, Chen Ye, Denghong Zuo, Luotao Wang, Xinyue Mei, Weiping Deng, Yixiang Liu, Huichuan Huang, Jianjun Hao, Jiangchao Zhao, Dongli Wang, Xin Zhang, Youyong Zhu, Junfeng Liu, Min Yang, Shusheng Zhu
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Abstract

Background: Plants thrive under biotic and abiotic stresses with the help of rhizomicrobiota. Root exudates play a pivotal role in recruiting beneficial microbes that assist plants in surviving environmental challenges, but the mechanisms of plant-microbiome interactions to resist multiple stresses remain elusive. We investigated how heat stress alters the rhizomicrobiomes of Panax notoginseng and how these heat stress-regulated microbes confer enhanced heat tolerance and disease resistance.

Results: We revealed that heat stress at 36 °C caused thermal damage to plants while enhancing heat tolerance and disease resistance for the survival of subsequent plants. Specifically, the beneficial microbes Burkholderia sp. and Saitozyma podzolica were recruited by the heat-stressed P. notoginseng and were confirmed to be responsible for resisting multiple stresses. Heat stress-induced plant roots secrete nucleotides such as purines and pyrimidines to promote the proliferation of these two beneficial microbes rather than root-rot pathogens. The exogenous application of these nucleotides to natural soil also resulted in the enrichment of the same beneficial microbes. Cross-species validation experiments in Capsicum annuum (pepper) and Solanum lycopersicum (tomato) further demonstrated that co-application of nucleotides with beneficial microbes synergistically enhanced heat tolerance.

Conclusions: Our findings highlight a plant strategy for thriving under multiple adversities and propose a potential pathway by leveraging nucleotide-mediated recruitment of beneficial microbes for enhancing plant resilience against multiple stresses. Video Abstract.

在热胁迫下富集的核苷酸招募有益的根际微生物来保护植物免受热和根腐病胁迫。
背景:植物在生物和非生物胁迫下都能在根状菌群的帮助下茁壮成长。根系分泌物在吸收有益微生物方面发挥着关键作用,帮助植物在环境挑战中生存,但植物-微生物相互作用抵御多种胁迫的机制尚不清楚。我们研究了热胁迫如何改变三七的根际微生物组,以及这些热胁迫调节的微生物如何增强耐热性和抗病性。结果:36°C的热胁迫对植株造成热损伤,但对后续植株的耐热性和抗病性有增强作用。具体来说,三七吸收了有益菌伯克霍尔德氏菌和灰土齐霉,证实了它们抵抗多种胁迫的作用。热胁迫诱导的植物根系分泌嘌呤和嘧啶等核苷酸,促进这两种有益微生物的增殖,而不是根腐病病原体。这些核苷酸外源应用于自然土壤也导致了相同的有益微生物的富集。辣椒(Capsicum annuum)和番茄(Solanum lycopersicum)的跨物种验证实验进一步证明,核苷酸与有益微生物的共同施用可协同增强耐热性。结论:我们的研究结果强调了植物在多种逆境下茁壮成长的策略,并提出了利用核苷酸介导的有益微生物招募来增强植物对多种逆境的抵御能力的潜在途径。视频摘要。
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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