Unlocking the potential of mitochondrial transplantation: overcoming challenges and paving the way for routine therapeutic application.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-08-01 Epub Date: 2025-07-05 DOI:10.1007/s10616-025-00805-8

Amaneh Mohammadi Roushandeh, Kazuo Tomita, Yoshikazu Kuwahara, Nima Najafi-Ghalehlou, Tomoaki Sato, Mehryar Habibi Roudkenar

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引用次数: 0

Abstract

Mitochondrial medicine has shown great promise as a therapeutic approach for treating currently incurable diseases. Preclinical studies highlight its safety and efficacy, but significant challenges remain in translating these therapies from bench to bedside. Key unresolved issues include understanding the mechanisms behind the reparative potential of transplanted mitochondria, such as their viability and functionality in an extracellular environment, especially under elevated calcium ion concentrations. Additionally, challenges related to mitochondrial sourcing, delivery methods, and ethical considerations need to be addressed for broader clinical adoption. This review analyses these challenges and explores strategies to overcome them, including refining mitochondrial sourcing, delivery techniques, and storage solutions. We also emphasise the need for rigorous ethical guidelines and regulatory frameworks to ensure safe and global implementation, paving the way for mitochondrial medicine's broader clinical use.

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释放线粒体移植的潜力：克服挑战，为常规治疗应用铺平道路。

线粒体医学作为一种治疗目前无法治愈的疾病的治疗方法已经显示出巨大的希望。临床前研究强调了其安全性和有效性，但在将这些疗法从实验室转化为临床方面仍存在重大挑战。尚未解决的关键问题包括了解移植线粒体修复潜力背后的机制，例如它们在细胞外环境中的生存能力和功能，特别是在钙离子浓度升高的情况下。此外，为了更广泛的临床应用，需要解决与线粒体来源、输送方法和伦理考虑相关的挑战。本文分析了这些挑战，并探讨了克服这些挑战的策略，包括改进线粒体来源、输送技术和储存解决方案。我们还强调需要严格的伦理准则和监管框架，以确保安全和全球实施，为线粒体医学更广泛的临床应用铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.