Liang Quan, Yuan Xin, Zhiling Zhang, Cheng Zhou, Qiang Ao
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引用次数: 0
Abstract
Bacterial resistance poses a serious clinical challenge. This study designs a peptide-enhanced bioactive hydrogel with a photodynamic-phage synergistic antibacterial effect that is an efficient antibacterial and promotes wound healing. LaFeO3@C3N4 is characterized with XRD, XPS, SEM, and UV-vis, and the photodynamic properties of LaFeO3@C3N4 producing ROS are confirmed by EPR. The peptide QHREDGS-modified boronate ester hydrogel is characterized by FT-IR, 1HNMR, SEM, dynamic mechanical analysis, etc. Combined LaFeO3@C3N4 and phage phipa10, the peptide QHREDGS-modified boronate ester hydrogel shows synergistic antibacterial and wound healing effects in both in vitro and in vivo experiments. Moreover, the molecular mechanism of tissue regeneration is explored by transcriptomic and protein expression analysis, and the results show that the peptide-enhanced bioactive hydrogel combined with photodynamic-phage synergistic antibacterial therapy system (QBC@DP-P-phi) significantly promoted cell proliferation and migration by regulating key signaling pathways such as PI3K-Akt, VEGF, and MAPK. Overall, this novel hydrogel is promising for the clinical treatment of infected wounds.
期刊介绍:
Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.