Peptide-Enhanced Bioactive Hydrogel Combined with Photodynamic-Phage Synergistic Antibacterial Therapy System Accelerates Infected Wound Healing.

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Liang Quan, Yuan Xin, Zhiling Zhang, Cheng Zhou, Qiang Ao
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引用次数: 0

Abstract

Bacterial resistance poses a serious clinical challenge. This study designs a peptide-enhanced bioactive hydrogel with a photodynamic-phage synergistic antibacterial effect that is an efficient antibacterial and promotes wound healing. LaFeO3@C3N4 is characterized with XRD, XPS, SEM, and UV-vis, and the photodynamic properties of LaFeO3@C3N4 producing ROS are confirmed by EPR. The peptide QHREDGS-modified boronate ester hydrogel is characterized by FT-IR, 1HNMR, SEM, dynamic mechanical analysis, etc. Combined LaFeO3@C3N4 and phage phipa10, the peptide QHREDGS-modified boronate ester hydrogel shows synergistic antibacterial and wound healing effects in both in vitro and in vivo experiments. Moreover, the molecular mechanism of tissue regeneration is explored by transcriptomic and protein expression analysis, and the results show that the peptide-enhanced bioactive hydrogel combined with photodynamic-phage synergistic antibacterial therapy system (QBC@DP-P-phi) significantly promoted cell proliferation and migration by regulating key signaling pathways such as PI3K-Akt, VEGF, and MAPK. Overall, this novel hydrogel is promising for the clinical treatment of infected wounds.

肽增强生物活性水凝胶联合光动力-噬菌体协同抗菌治疗系统加速感染伤口愈合。
细菌耐药性构成了严重的临床挑战。本研究设计了一种多肽增强生物活性水凝胶,具有光动力-噬菌体协同抗菌作用,是一种有效的抗菌药物,促进伤口愈合。通过XRD、XPS、SEM和UV-vis对LaFeO3@C3N4进行了表征,并通过EPR证实了LaFeO3@C3N4产生ROS的光动力学性质。采用FT-IR、1HNMR、SEM、动态力学分析等手段对qhredgs修饰的硼酸酯水凝胶进行了表征。结合LaFeO3@C3N4和噬菌体phipa10,肽qhredgs修饰的硼酸酯水凝胶在体外和体内实验中均显示出协同抗菌和伤口愈合的作用。此外,通过转录组学和蛋白表达分析探索组织再生的分子机制,结果表明肽增强生物活性水凝胶联合光动力-噬菌体协同抗菌治疗系统(QBC@DP-P-phi)通过调节PI3K-Akt、VEGF、MAPK等关键信号通路,显著促进细胞增殖和迁移。总之,这种新型水凝胶在感染伤口的临床治疗中具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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