Microbial biomass conversion for hydrogen production: A review

Abstract

The escalating demand for clean and sustainable energy sources has propelled hydrogen to the forefront of alternative fuel research. Microbial biomass conversion, a bio-based process utilizing microorganisms to convert organic matter into hydrogen, presents a promising avenue for achieving this goal. This review provides a comprehensive overview of possible microbial biomass conversion methods, including both light-dependent and light-independent methods, and compares their hydrogen production rates (HPRs). Light-dependent methods such as photo-fermentation offer HPRs exceeding 3 m³/dm³, suggesting highly efficient hydrogen generation possibilities. However, most rely on indirect processes or specific light conditions, potentially hindering H₂ production. Dark fermentation (DF) demonstrates significantly higher HPRs, up to 12 m³/d/m³, with no light requirements, making it a strong contender for large-scale production. Microbial electrolysis cells (MECs) show even greater HPRs of up to 72 m³/d/m³, competing favorably in hydrogen generation feasibility. Despite promising advancements, challenges remain in scaling up these processes for commercial viability. While current research achieves high HPRs, reactor volumes are typically below 1 L. This review explores opportunities and challenges associated with scaling up, particularly focusing on integrating DF and MECs. Combining these methods holds promise for enhancing stability and achieving efficient energy recovery.