Establishment of the NUMNi001-A human iPSC line from peripheral blood mononuclear cells of a healthy male donor

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-03 DOI:10.1016/j.scr.2025.103765

Akihito Tanaka , Kazuhiro Furuhashi , Jun Matsumoto , Keita Hattori , Akiko Owaki , Tomohiro Kawazoe , Akihisa Kato , Chikao Onogi , Yu Watanabe , Eri Koshi-Ito , Itaru Kushima , Norio Ozaki , Shoichi Maruyama

引用次数: 0

Abstract

Using an integration-free episomal vector containing the reprogramming components hOCT3/4, hSox2/KLF4, hL-MYC/LIN28, mp53DD, and EBNA-1, peripheral blood mononuclear cells obtained from a healthy 42-year-old man were successfully reprogrammed into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were cultured without feeder cells. They exhibited a normal karyotype, expressed pluripotency markers, and differentiated into cells representing all three germ layers. The NUMNi001-A line could serve as a control for investigating disease mechanisms.

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从健康男性供体外周血单个核细胞中建立NUMNi001-A人类多能干细胞系

利用含重编程成分hOCT3/4、hSox2/KLF4、hL-MYC/LIN28、mp53DD和EBNA-1的无整合外源载体，成功地将来自42岁健康男性的外周血单个核细胞重编程为诱导多能干细胞（iPSCs）。重新编程的iPSCs在没有饲养细胞的情况下培养。他们表现出正常的核型，表达多能性标记，并分化成代表所有三种胚层的细胞。NUMNi001-A系可以作为研究疾病机制的对照。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.