Gibberellic acid-mediated transcriptional divergence underlies cold stress adaptation in two diploid cotton species.

IF 2.3 3区 生物学 Q2 MULTIDISCIPLINARY SCIENCES
PeerJ Pub Date : 2025-07-03 eCollection Date: 2025-01-01 DOI:10.7717/peerj.19721
Dong Wang, Juyun Zheng, Ke Liu, Yanchao Xu, Dingsha Jin
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引用次数: 0

Abstract

Background: The diploid cotton species Gossypium thurberi (D1) and Gossypium trilobum (D8) exhibit significant divergence in cold stress tolerance despite their close phylogenetic relationship.

Methods: To explore the genetic basis of this difference, we conducted a comparative transcriptomic analysis under cold stress at 4 °C, identifying 697 and 311 species-specific differentially expressed genes (DEGs) in G. thurberi and G. trilobum, respectively. Functional enrichment analysis was performed to investigate the biological pathways associated with these DEGs. Additionally, hormone levels, particularly gibberellic acid (GA), were measured to assess their role in cold stress responses.

Results: The DEGs in both species were significantly enriched in the "hormone signal transduction" pathway, highlighting the importance of hormonal regulation in cold adaptation. Distinct trends in GA levels were observed between G. thurberi and G. trilobum, with GA strongly correlated with species-specific DEGs. G. thurberi demonstrated greater cold tolerance than G. trilobum, likely due to a more robust GA-regulated response.

Conclusion: These findings indicate that expression divergence in GA-mediated pathways between sister species has driven adaptive evolution in cold stress tolerance. This study not only advances our understanding of cold adaptation mechanisms in cotton but also provides genetic insights for improving cold tolerance in cultivated varieties through targeted breeding and genetic engineering.

赤霉素介导的转录分化是两种二倍体棉花适应冷胁迫的基础。
背景:二倍体棉种Gossypium thurberi (D1)和Gossypium trilobum (D8)尽管亲缘关系密切,但在冷胁迫耐受性方面存在显著差异。方法:为了探究这一差异的遗传基础,我们在4°C低温胁迫下进行了转录组学比较分析,分别鉴定了红毛茛和三叶毛茛中697个和311个种特异性差异表达基因(DEGs)。功能富集分析研究了与这些deg相关的生物学途径。此外,还测量了激素水平,特别是赤霉素(GA),以评估它们在冷应激反应中的作用。结果:两种植物的deg在“激素信号转导”通路中均显著富集,表明激素调节在冷适应中的重要性。赤霉素和三叶赤霉素的赤霉素含量变化趋势明显,赤霉素与种特异性赤霉素含量密切相关。thurberi表现出比trilobum更强的耐寒性,可能是由于ga调控的反应更强。结论:这些研究结果表明,姐妹种之间ga介导通路的表达差异推动了冷胁迫耐受性的适应性进化。该研究不仅有助于进一步了解棉花的冷适应机制,而且为通过定向育种和基因工程提高栽培品种的耐寒性提供了遗传学见解。
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来源期刊
PeerJ
PeerJ MULTIDISCIPLINARY SCIENCES-
CiteScore
4.70
自引率
3.70%
发文量
1665
审稿时长
10 weeks
期刊介绍: PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.
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