Assessment of antifungal and anti-biofilm potential of essential oil active constituents alone and in combinatorial mode of limonene and linalool against Candida albicans and Candida tropicalis.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-07-07 DOI:10.1080/14786419.2025.2526731

Pankaj Kumar Chaudhary, Deepika Saini, Amit Kumar Srivastava, Ramasare Prasad

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引用次数: 0

Abstract

Opportunistic Candidiasis is rising among immunocompromised and elderly patients, with increasing antifungal resistance. This study evaluated five essential oil-derived compounds against Candida albicans and Candida tropicalis. Limonene showed the strongest antifungal activity, with minimum inhibitory concentration (MICs) of 32-64 µg/mL. Similarly, biofilm inhibition/eradication concentration (BIC/BEC) was found in followed the order: limonene > linalool > 1,8-cineole = 2-undecanone = borneol. Notably, the combination of limonene and linalool showed additive effects, with fractional inhibitory concentration index (FICI) values of 0.79 for C. albicans and 0.98 for C. tropicalis. This combinatorial approach, investigated for the first time, showed no significant cytotoxicity on human embryonic kidney (HEK-293) and human keratinocyte (HaCaT) cell lines. A co-culture model of Candida spp. and HaCaT cells was developed to study host-pathogen interactions and antifungal efficacy. Results highlight EO-ACs, especially limonene and linalool, as promising alternatives against resistant Candida infections.

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精油活性成分单独及柠檬烯和芳樟醇联合抗白色念珠菌和热带念珠菌的抗真菌和抗生物膜潜力评价。

机会性念珠菌病在免疫功能低下和老年患者中呈上升趋势，抗真菌耐药性增加。本研究评价了5种精油衍生化合物对白色念珠菌和热带念珠菌的抑制作用。柠檬烯的抑菌活性最强，最低抑菌浓度（mic）为32 ~ 64µg/mL。同样，生物膜抑制/根除浓度（BIC/BEC）的大小顺序为：柠檬烯>芳樟醇> 1,8-桉叶脑= 2-烯酮=冰片。值得注意的是，柠檬烯与芳樟醇联合使用对白念珠菌的分数抑制浓度指数（FICI）为0.79，对热带念珠菌的分数抑制浓度指数为0.98。首次研究的这种组合方法对人胚胎肾（HEK-293）和人角化细胞（HaCaT）细胞系没有明显的细胞毒性。建立假丝酵母和HaCaT细胞共培养模型，研究宿主-病原体相互作用和抗真菌效果。结果强调eo - ac，特别是柠檬烯和芳樟醇，是抗耐药念珠菌感染的有希望的替代品。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.