{"title":"Improved Lovastatin Production via Targeted Medium Supplementation in Aspergillus terreus.","authors":"Maryam Javanpour, Ramtin Vamenani, Ethan Brister","doi":"10.1002/bab.70025","DOIUrl":null,"url":null,"abstract":"<p><p>Lovastatin is a clinically crucial cholesterol-lowering agent produced by Aspergillus terreus; however, its commercial biosynthesis remains limited due to suboptimal fermentation conditions. This study addresses the challenge of evaluating how the supplementation of l-cysteine (l-Cys), linoleic acid (LA), and riboflavin (vitamin B2) individually, in dual combinations, and, as a triple formulation, affects both fungal biomass and lovastatin yield. Among individual supplements, LA had the most pronounced effect, increasing lovastatin production to 1892.43 mg/L, whereas l-Cys and B2 yielded 1600.66 and 1176.82 mg/L, respectively. Dual combinations, such as l-Cys + LA and LA + B2, produced higher titers (2300 and 2282.96 mg/L, respectively), leading to significant increases in yield per gram of biomass. The highest productivity was achieved with triple supplementation (115 mg/L l-Cys + 90 mg/L LA + 0.66 mg/L B2), resulting in 2373.99 mg/L lovastatin, a biomass of 8.03 g/L, and a yield of 295.65 mg/g biomass. Biomass measurements across all treatments indicated that although fungal growth was moderately enhanced, the increase in lovastatin production was disproportionately greater. This suggests that the supplements primarily stimulate metabolic flux toward polyketide synthesis. These findings provide a foundation for media optimization strategies to enhance industrial-scale lovastatin production through precise nutrient control.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and applied biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/bab.70025","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Lovastatin is a clinically crucial cholesterol-lowering agent produced by Aspergillus terreus; however, its commercial biosynthesis remains limited due to suboptimal fermentation conditions. This study addresses the challenge of evaluating how the supplementation of l-cysteine (l-Cys), linoleic acid (LA), and riboflavin (vitamin B2) individually, in dual combinations, and, as a triple formulation, affects both fungal biomass and lovastatin yield. Among individual supplements, LA had the most pronounced effect, increasing lovastatin production to 1892.43 mg/L, whereas l-Cys and B2 yielded 1600.66 and 1176.82 mg/L, respectively. Dual combinations, such as l-Cys + LA and LA + B2, produced higher titers (2300 and 2282.96 mg/L, respectively), leading to significant increases in yield per gram of biomass. The highest productivity was achieved with triple supplementation (115 mg/L l-Cys + 90 mg/L LA + 0.66 mg/L B2), resulting in 2373.99 mg/L lovastatin, a biomass of 8.03 g/L, and a yield of 295.65 mg/g biomass. Biomass measurements across all treatments indicated that although fungal growth was moderately enhanced, the increase in lovastatin production was disproportionately greater. This suggests that the supplements primarily stimulate metabolic flux toward polyketide synthesis. These findings provide a foundation for media optimization strategies to enhance industrial-scale lovastatin production through precise nutrient control.
期刊介绍:
Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation.
The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.