Antimigratory, Anti-Invasive and Anti-Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β-Catenin Pathway

IF 2 4区医学 Q2 RHEUMATOLOGY

International Journal of Rheumatic Diseases Pub Date : 2025-07-07 DOI:10.1111/1756-185x.70360

Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi

{"title":"Antimigratory, Anti-Invasive and Anti-Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β-Catenin Pathway","authors":"Haoyu Yang, Xunhao Wang, Jiayan Zhu, Yuzhou Liu, Gang Zhao, Jingyi Mi","doi":"10.1111/1756-185x.70360","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. In vivo, Bergenin alleviated paw swelling, arthritis score, and synovial pathology in AIA rats, reducing pro-inflammatory factors.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Bergenin exerts antimigratory, anti-invasive, and anti-inflammatory effects in rheumatoid arthritis by modulating the Wnt/β-catenin pathway, suggesting its potential as a therapeutic option for rheumatoid arthritis.</p>\n </section>\n </div>","PeriodicalId":14330,"journal":{"name":"International Journal of Rheumatic Diseases","volume":"28 7","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Rheumatic Diseases","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1756-185x.70360","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

This study investigates antimigratory, anti-invasive, and anti-inflammatory mechanisms of Bergenin in patients with rheumatoid arthritis (RA) by focusing on the pathways involved.

Methods

In vitro, TNF-α-induced MH7A cells were used to assess Bergenin's effects on cell proliferation (CCK-8), migration (wound healing, Transwell assays), and apoptosis (flow cytometry). mRNA levels of IL-6 and MMPs were quantified by qRT-PCR, and protein expression was analyzed using ELISA and western blot. The Wnt/β-catenin pathway was examined through immunofluorescence and western blotting with or without LiCl. An adjuvant-induced arthritis (AIA) rat model was used to monitor clinical symptoms and analyze synovial tissue.

Results

Bergenin inhibited the proliferation, migration, and invasion of TNF-α-induced MH7A cells, while promoting apoptosis. It reduced IL-6 and MMPs expression, with these effects reversed by LiCl. In vivo, Bergenin alleviated paw swelling, arthritis score, and synovial pathology in AIA rats, reducing pro-inflammatory factors.

Conclusions

Bergenin exerts antimigratory, anti-invasive, and anti-inflammatory effects in rheumatoid arthritis by modulating the Wnt/β-catenin pathway, suggesting its potential as a therapeutic option for rheumatoid arthritis.

查看原文本刊更多论文

甜菜素通过下调Wnt/β-Catenin通路对类风湿关节炎的抗迁移、抗侵袭和抗炎作用

本研究通过对相关通路的研究，探讨了卑尔根素在类风湿关节炎（RA）患者中的抗迁移、抗侵袭和抗炎机制。方法采用TNF-α-诱导的MH7A细胞体外实验，评估甜菜根素对细胞增殖（CCK-8）、迁移（Transwell实验）和凋亡（流式细胞术）的影响。采用qRT-PCR检测IL-6和MMPs mRNA水平，ELISA和western blot检测蛋白表达情况。通过免疫荧光和western blot检测Wnt/β-catenin通路。采用佐剂性关节炎（AIA）大鼠模型监测临床症状并分析滑膜组织。结果龙根素能抑制TNF-α-诱导的MH7A细胞的增殖、迁移和侵袭，促进细胞凋亡。它降低了IL-6和MMPs的表达，而这些作用被LiCl逆转。在体内，甜菜根素减轻了AIA大鼠的足跖肿胀、关节炎评分和滑膜病理，降低了促炎因子。结论卑尔根素通过调节Wnt/β-catenin通路，在类风湿关节炎中发挥抗迁移性、抗侵袭性和抗炎作用，提示其可能作为类风湿关节炎的治疗选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Rheumatic Diseases RHEUMATOLOGY-

CiteScore

3.70

自引率

4.00%

发文量

362

审稿时长

1 months

期刊介绍： The International Journal of Rheumatic Diseases (formerly APLAR Journal of Rheumatology) is the official journal of the Asia Pacific League of Associations for Rheumatology. The Journal accepts original articles on clinical or experimental research pertinent to the rheumatic diseases, work on connective tissue diseases and other immune and allergic disorders. The acceptance criteria for all papers are the quality and originality of the research and its significance to our readership. Except where otherwise stated, manuscripts are peer reviewed by two anonymous reviewers and the Editor.