Breaking barriers in soft material printing: High-fidelity DLP fabrication of silk sericin via height-adjusted curing and local error optimization

IF 11.1 1区 工程技术 Q1 ENGINEERING, MANUFACTURING
Xuanwen Wang, Xiaoliang Cui, Hui Wang, Jun Zhang, Ke-Qin Zhang
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Abstract

Digital Light Processing (DLP) faces significant challenges in printing ultra-soft hydrogels due to their weak mechanical properties and the lack of robust accuracy evaluation systems. Here, we overcome these limitations by introducing silk sericin (SS) - a protein recovered from alkaline thermal degumming water - as a sustainable bioink. Through glycidyl methacrylate (GMA) modification, we developed methacrylated silk sericin (SerMA) with enhanced curability while preserving its inherent biocompatibility. A "Height Gradient Screening" approach was innovatively applied to the Jacob's working curve, enabling precise determination of cured depth under real-world printing conditions by addressing light scattering and self-focusing effects. Furthermore, we proposed the "Tangent Cylindrical Model", a novel framework to quantify localized printing errors across dynamic illumination source spacings, achieving resolutions as fine as 300 µm channel gaps and 400 µm hole structures-surpassing current capabilities for weak hydrogels. The printed SerMA constructs demonstrated excellent cytocompatibility (L929 cell viability>95 % over 168 h) and supported cell adhesion/spreading, validated via Live/Dead assays and F-actin staining. This work not only expands the DLP material library to include recovered, mechanically fragile proteins but also establishes a universal methodology for high-precision printing of soft biomaterials, with direct implications for tissue engineering, microfluidics, and biosensing.
突破软质材料印刷的障碍:通过高度调节固化和局部误差优化的丝胶高保真DLP制造
数字光处理(DLP)在打印超软水凝胶方面面临着巨大的挑战,因为它们的机械性能较弱,而且缺乏可靠的精度评估系统。在这里,我们克服了这些限制,引入丝胶蛋白(SS)——一种从碱性热脱胶水中回收的蛋白质——作为一种可持续的生物链接。通过甲基丙烯酸缩水甘油酯(GMA)改性,制备出具有增强固化性的甲基丙烯酸丝胶(SerMA),同时保留了其固有的生物相容性。“高度梯度筛选”方法创新地应用于雅各布的工作曲线,通过解决光散射和自聚焦效应,能够在实际打印条件下精确确定固化深度。此外,我们提出了“切线圆柱形模型”,这是一个新的框架,用于量化动态照明源间隔的局部打印误差,实现了300 µm通道间隙和400 µm孔结构的分辨率,超过了目前弱水凝胶的能力。通过活/死实验和F-actin染色证实,打印的SerMA结构具有出色的细胞相容性(在168 h内L929细胞存活率>; 95% %)和支持细胞粘附/扩散。这项工作不仅扩展了DLP材料库,包括恢复的、机械脆弱的蛋白质,而且还建立了一种用于高精度打印软生物材料的通用方法,对组织工程、微流体和生物传感具有直接意义。
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来源期刊
Additive manufacturing
Additive manufacturing Materials Science-General Materials Science
CiteScore
19.80
自引率
12.70%
发文量
648
审稿时长
35 days
期刊介绍: Additive Manufacturing stands as a peer-reviewed journal dedicated to delivering high-quality research papers and reviews in the field of additive manufacturing, serving both academia and industry leaders. The journal's objective is to recognize the innovative essence of additive manufacturing and its diverse applications, providing a comprehensive overview of current developments and future prospects. The transformative potential of additive manufacturing technologies in product design and manufacturing is poised to disrupt traditional approaches. In response to this paradigm shift, a distinctive and comprehensive publication outlet was essential. Additive Manufacturing fulfills this need, offering a platform for engineers, materials scientists, and practitioners across academia and various industries to document and share innovations in these evolving technologies.
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