Metabolic pathway modulation for enhanced acetic acid production in acidogenic fermentation of food waste via gas self-circulation

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-07-07 DOI:10.1016/j.biortech.2025.132945

Yonghui Zheng , Penghui Chen , Enzhen Wang , Yuying Ren , Xingguo Wu , Quanyuan Wei , Renjie Dong , Jianbin Guo

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Abstract

The volatile fatty acids (VFAs) produced through acidogenic fermentation of food waste can be applied as an external carbon source for denitrification in wastewater treatment. However, variations in the concentration and composition of VFAs significantly limit denitrification performance. In this study, a novel gas self-circulation strategy was investigated to enhance acetic acid production. The results indicated that gas self-circulation increased the acetic acid yield by 23.11 %, from 6.88 to 8.47 g/L. Metagenomic analyses revealed that the system reduced reliance on conventional acetogenic bacteria and significantly enriched homoacetogens. The upregulation of genes related to glycolysis and pyruvate metabolism explained the overall improvement in acidogenic metabolism. The increased abundance of key enzyme genes associated with the Wood–Ljungdahl and the acetyl coenzyme A pathways enhanced the metabolic flux toward acetate synthesis. This study presents a novel approach for enhancing selective acetic acid production and investigates the corresponding mechanistic responses.

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食物垃圾产酸发酵过程中通过气体自循环提高乙酸产量的代谢途径调节

食物垃圾产酸发酵产生的挥发性脂肪酸（VFAs）可以作为废水处理中反硝化的外部碳源。然而，VFAs浓度和组成的变化显著限制了反硝化性能。在本研究中，研究了一种新的气体自循环策略，以提高乙酸的产量。结果表明，气体自循环使乙酸产率由6.88 g/L提高到8.47 g/L，提高了23.11%。宏基因组分析显示，该系统减少了对传统产醋菌的依赖，并显著富集了同质产醋菌。糖酵解和丙酮酸代谢相关基因的上调解释了产酸代谢的整体改善。与Wood-Ljungdahl和乙酰辅酶A途径相关的关键酶基因丰度的增加增强了乙酸合成的代谢通量。本研究提出了一种提高选择性醋酸生产的新方法，并探讨了相应的机制反应。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.