Effect of preservation techniques on the phytochemical profile, antioxidant activity, and glucose adsorption capacity of oyster mushrooms (Pleurotus ostreatus)

IF 2.7 3区生物学 Q2 PLANT SCIENCES

South African Journal of Botany Pub Date : 2025-07-07 DOI:10.1016/j.sajb.2025.06.042

Oluwole Solomon Oladeji , Farhan Mohd Said , Nur Fathin Shamirah Daud , Siti Aisyah Nabila Mohd Hosnee , Natrah Atiqah Maspan , Nur Syafiqah Zakuan , Noor Hasniza Md Zin , Fathie Ahmad Zakil

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引用次数: 0

Abstract

The growing demand for foods with enhanced shelf life and nutritional value has driven the rapid expansion of processed food products, often outpacing natural alternatives. This study explores the effects of oven-drying, salt-drying, and freeze-drying on Pleurotus ostreatus (Oyster mushrooms) cultivated on oil palm frond substrates. Key parameters analysed include moisture content (MC) and protein content (PC), total phenolic content (TPC), antioxidant activity, glucose adsorption capacity (GAC), and elemental composition. CHNS analysis confirmed the presence of carbon, hydrogen, nitrogen, and sulphur in all substrates, while MC varied slightly (82–88.54 %) with substrate composition. Protein analysis revealed that freeze-dried samples had the highest PC, while SDS-PAGE profiling showed protein bands (35–75 kDa) in all samples except salt-treated ones, which were denatured. Over 60 days, oven-dried substrates maintained relatively high PC (∼4–5 mg/ml), whereas freeze-dried samples exhibited a significant decline. Salt-treated and oven-dried samples extracted with phosphate buffer consistently showed low PC. GAC increased with molar concentration, with oven-dried samples exhibiting the highest efficiency. Regarding antioxidant properties, salt-treated samples demonstrated increased free-radical scavenging activity after storage, while oven-dried and freeze-dried samples showed a decline. TPC decreased over time in all treatments, but oven-dried samples gradually increased, particularly in substrate III (87.5 %). These findings suggest that preservation methods effectively extend shelf life while maintaining bioactive compounds that may influence glucose absorption and the potential anti-diabetic properties of P. ostreatus. Further research should explore sensory attributes, toxicological safety, and in vivo molecular mechanisms.

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保鲜技术对平菇（Pleurotus ostreatus）植物化学特征、抗氧化活性和葡萄糖吸附能力的影响

对保质期更长、营养价值更高的食品的需求不断增长，推动了加工食品的迅速扩张，往往超过了天然替代品。本研究探讨了烘箱干燥、盐干燥和冷冻干燥对油棕叶基质上栽培的平菇的影响。分析的关键参数包括水分含量（MC）和蛋白质含量（PC）、总酚含量（TPC）、抗氧化活性、葡萄糖吸附能力（GAC）和元素组成。CHNS分析证实，所有底物中均存在碳、氢、氮和硫，而MC随底物组成略有变化（82 - 88.54%）。蛋白质分析显示，冻干样品的PC最高，而SDS-PAGE分析显示，除盐处理后的样品变性外，所有样品的蛋白质条带（35-75 kDa）均为蛋白质条带。在60天内，烘箱干燥的底物保持相对较高的PC (~ 4-5 mg/ml)，而冻干的样品则明显下降。磷酸盐缓冲液提取的盐处理和烘箱干燥样品一致显示低PC。GAC随摩尔浓度的增加而增加，其中烘箱干燥样品的效率最高。在抗氧化性能方面，盐处理后的样品在储存后显示出增加的自由基清除活性，而烘箱干燥和冷冻干燥的样品则显示出下降。在所有处理中，TPC随着时间的推移而下降，但烘干样品逐渐增加，特别是在底物III中（87.5%）。这些发现表明，保存方法有效地延长了保质期，同时保留了可能影响葡萄糖吸收和潜在抗糖尿病特性的生物活性化合物。进一步的研究应探讨感官特性、毒理学安全性和体内分子机制。

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来源期刊

South African Journal of Botany 生物-植物科学

CiteScore

5.20

自引率

9.70%

发文量

709

审稿时长

61 days

期刊介绍： The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.