Bioprocess development for the production of poly(3-hydroxybutyrate) using the invasive macroalgae Rugulopteryx okamurae

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation Pub Date : 2025-07-02 DOI:10.1016/j.eti.2025.104342

Cristina Agabo-García , Mirva Sarafidou , Olga Psaki , Chrysanthi Argeiti , Ana Blandino , Carlos Álvarez-Gallego , Luis Isidoro Romero-García , Apostolis Koutinas

{"title":"Bioprocess development for the production of poly(3-hydroxybutyrate) using the invasive macroalgae Rugulopteryx okamurae","authors":"Cristina Agabo-García , Mirva Sarafidou , Olga Psaki , Chrysanthi Argeiti , Ana Blandino , Carlos Álvarez-Gallego , Luis Isidoro Romero-García , Apostolis Koutinas","doi":"10.1016/j.eti.2025.104342","DOIUrl":null,"url":null,"abstract":"<div><div>The brown macroalga Rugulopteryx okamurae was used to produce a sugar-rich hydrolysate, which served as a carbon source in Paraburkholderia sacchari DSM 17165 cultures for the production of poly(3-hydroxybutyrate) (PHB). Biological pretreatment of R. okamurae using the fungal strains Aspergillus awamori and Aspergillus oryzae in solid-state fermentation (SSF) was employed to produce alginate lyase (24 IU/gbiomass) and protease (20 IU/gbiomass). The fermented solids were used for enzymatic hydrolysis in combination with a commercial cocktail of enzymes containing cellulase and hemicellulase. SSF of the macroalgae for 72 h by A. oryzae, followed by enzymatic saccharification for 48 h, resulted in a total reducing sugars (TRS) concentration of around 120 mgTRS/gbiomass. Conducting a bioreactor batch fermentation on macroalgae-derived hydrolysate for PHB production by P. sacchari, consuming 51 g/L of glucose, resulted in 43.3 g/L of dry cell weight with 28.4 % PHB content (12.3 g/L PHB). This study demonstrated the feasibility of exploiting macroalgae as a feedstock for PHB production.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104342"},"PeriodicalIF":6.7000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Technology & Innovation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352186425003281","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The brown macroalga Rugulopteryx okamurae was used to produce a sugar-rich hydrolysate, which served as a carbon source in Paraburkholderia sacchari DSM 17165 cultures for the production of poly(3-hydroxybutyrate) (PHB). Biological pretreatment of R. okamurae using the fungal strains Aspergillus awamori and Aspergillus oryzae in solid-state fermentation (SSF) was employed to produce alginate lyase (24 IU/g_biomass) and protease (20 IU/g_biomass). The fermented solids were used for enzymatic hydrolysis in combination with a commercial cocktail of enzymes containing cellulase and hemicellulase. SSF of the macroalgae for 72 h by A. oryzae, followed by enzymatic saccharification for 48 h, resulted in a total reducing sugars (TRS) concentration of around 120 mg_TRS/g_biomass. Conducting a bioreactor batch fermentation on macroalgae-derived hydrolysate for PHB production by P. sacchari, consuming 51 g/L of glucose, resulted in 43.3 g/L of dry cell weight with 28.4 % PHB content (12.3 g/L PHB). This study demonstrated the feasibility of exploiting macroalgae as a feedstock for PHB production.

查看原文本刊更多论文

利用入侵大藻玉隼生产聚（3-羟基丁酸酯）的生物工艺研究

利用褐藻Rugulopteryx okamurae生产富含糖的水解产物，作为Paraburkholderia sacchari DSM 17165培养物的碳源，用于生产聚（3-羟基丁酸盐）（PHB）。采用固体发酵法对冈村黑僵菌进行生物预处理，得到海藻酸解酶（24 IU/gbiomass）和蛋白酶（20 IU/gbiomass）。发酵固体与含有纤维素酶和半纤维素酶的商业混合酶一起用于酶水解。a.m oryzae对大型藻类进行72 h的SSF，然后进行48 h的酶解糖化，总还原糖（TRS）浓度约为120 mgTRS/gbiomass。利用大藻衍生的水解液进行生物反应器间歇发酵，通过p.s chari生产PHB，消耗51 g/L葡萄糖，得到43.3 g/L的干细胞重量，28.4 %的PHB含量（12.3 g/L PHB）。本研究证明了利用大型藻类作为PHB生产原料的可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Environmental Technology & Innovation Environmental Science-General Environmental Science

CiteScore

14.00

自引率

4.20%

发文量

435

审稿时长

74 days

期刊介绍： Environmental Technology & Innovation adopts a challenge-oriented approach to solutions by integrating natural sciences to promote a sustainable future. The journal aims to foster the creation and development of innovative products, technologies, and ideas that enhance the environment, with impacts across soil, air, water, and food in rural and urban areas. As a platform for disseminating scientific evidence for environmental protection and sustainable development, the journal emphasizes fundamental science, methodologies, tools, techniques, and policy considerations. It emphasizes the importance of science and technology in environmental benefits, including smarter, cleaner technologies for environmental protection, more efficient resource processing methods, and the evidence supporting their effectiveness.