Diagnostic utility of multiplex PCR for direct detection and serotyping of group B streptococci from clinical specimens

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-07-05 DOI:10.1016/j.mimet.2025.107190

Muhammed Basith Hafeez Ismail, Geetha Nagaraj, Kadahalli Lingegowda Ravikumar

引用次数: 0

Abstract

Multiplex PCR analysis of 348 recto-vaginal swabs from non-pregnant women revealed 33 % GBS colonization, with serotype V predominant. The study highlights the utility of multiplex PCR as a rapid and accurate diagnostic tool for detecting and serotyping Group B Streptococci directly from clinical specimens, supporting its role in surveillance, infection control, and guiding vaccine strategies in this underrepresented population.

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多重PCR对临床标本中B群链球菌直接检测及血清分型的诊断价值

对348份非孕妇直肠阴道拭子的多重PCR分析显示，33%的GBS定植，以血清V型为主。该研究强调多重PCR作为一种快速准确的诊断工具的实用性，可直接从临床标本中检测和分型B群链球菌，支持其在这一代表性不足的人群中的监测、感染控制和指导疫苗策略方面的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.