Exosomal tpi-miR-10a-5p from T. pisiformis cysticerci regulates the expression of inflammatory factors in rabbits by targeting MAP3K7

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-07-04 DOI:10.1016/j.vetpar.2025.110537

Guiting Pu , Liqun Wang , Tingli Liu , Dexian Wang , Hong Li , Tharheer Oluwashola Amuda , Hong Yin , Hongbin Yan , Xueyong Zhang , Xuenong Luo

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引用次数: 0

Abstract

Taenia pisiformis (T. pisiformis) cysticerci, belonging to Taeniidae, attaches to the wall of the mesentery and omentum of rabbits, causing cysticercosis pisiformis that can seriously affect the healthy development of the rabbit breeding industry. Helminths can produce exosomes containing proteins and RNAs. In our previous study, tpi-miR-10a-5p was found to be highly enriched in the exosomes from T. pisiformis cysticerci. In this study, we report that tpi-miR-10a-5p is significantly up-regulated in the blood and peripheral blood lymphocytes (PBLCs) of T. pisiformis-infected rabbits. Furthermore, tpi-miR-10a-5p targets mitogen-activated protein kinase kinase kinase 7 (MAP3K7), the key gene involved in the c-Jun N-terminal kinase (JNK) signaling pathway. Knockdown of MAP3K7 inhibited the JNK signaling pathway, suppressing the production of inflammatory cytokines such as IFN-γ and TNFα, whereas overexpression of MAP3K7 activated the JNK signaling pathway in PBLCs. The same trend was observed with knockdown of MAP3K7 when PBLCs were treated with exosomes from T. pisiformis cysticerci. In vivo experiment further demonstrated that the expression of MAP3K7, JNK, p-JNK, IFN-γ, and TNFα was significantly decreased in PBLCs during T. pisiformis infection. Therefore, tpi-miR-10a-5p can suppress the JNK signaling pathway and inflammatory response by targeting MAP3K7 in host PBLCs. These findings may imply a mechanism used by the parasites releasing exosomes to sense and adapt to the host environment by regulating the immune reaction.

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pisiformis囊虫外泌体tpi-miR-10a-5p通过靶向MAP3K7调控家兔炎症因子的表达

猪形带绦虫（T. pisiformis）囊尾蚴属于带绦虫科，附着在家兔肠系膜和网膜壁上，引起猪形带绦虫病，严重影响家兔养殖业的健康发展。蠕虫可以产生含有蛋白质和rna的外泌体。在我们之前的研究中，我们发现pisiformis囊虫的外泌体中高度富集tpi-miR-10a-5p。在本研究中，我们报道了tpi-miR-10a-5p在感染pisiformis的家兔血液和外周血淋巴细胞（PBLCs）中显著上调。此外，tpi-miR-10a-5p靶向丝裂原活化蛋白激酶激酶激酶7 (MAP3K7)，这是参与c-Jun n-末端激酶（JNK）信号通路的关键基因。MAP3K7的下调抑制了JNK信号通路，抑制了IFN-γ和tnf - α等炎症细胞因子的产生，而MAP3K7的过表达激活了PBLCs中的JNK信号通路。当PBLCs被pisiformis囊尾蚴外泌体处理时，MAP3K7的敲低也出现了同样的趋势。体内实验进一步证实，PBLCs中MAP3K7、JNK、p-JNK、IFN-γ和TNFα的表达在PBLCs感染后显著降低。因此，tpi-miR-10a-5p可以通过靶向宿主PBLCs中的MAP3K7来抑制JNK信号通路和炎症反应。这些发现可能暗示寄生虫释放外泌体通过调节免疫反应来感知和适应宿主环境的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.