CRISPR-based fluorescent aptasensor combined with smartphone for on-site visual detection of DEHP in packaged foods

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-07-04 DOI:10.1016/j.saa.2025.126649

Anjie Guo , Yu Wu , Yunkai Xie , Wang Guo , Yiqing Guo , Xinai Zhang , Wen Zhang , Xiaobo Zou , Zongbao Sun

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引用次数: 0

Abstract

Di-(2-ethylhexyl) phthalate (DEHP) can leach into food and the environment, posing health and ecological risks. This paper introduces a novel CRISPR-based fluorescent aptasensor for on-site DEHP detection. The aptasensor selectively binds to DEHP, triggering a competitive displacement reaction that releases an Aptamer-dsDNA probe. After magnetic separation, the supernatant is analyzed via a Cas12a reporter system, where Cas12a activation cleaves a FAM-BHQ reporter, generating fluorescence. Combined with smartphone imaging, this method enables rapid result acquisition. The aptasensor shows high selectivity and sensitivity, detecting DEHP from 1 pg/mL to 1 μg/mL, with a lower limit of 0.15 pg/mL. It effectively detects DEHP in various real samples, offering reliable visual monitoring. This method offers a rapid and effective on-site detection strategy for food safety and environmental pollution monitoring.

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基于crispr的荧光体传感器与智能手机相结合，用于包装食品中DEHP的现场视觉检测

邻苯二甲酸二（2-乙基己基）酯（DEHP）可渗入食物和环境，对健康和生态构成威胁。本文介绍了一种新型的基于crispr的DEHP现场检测荧光适体传感器。适配体传感器选择性地与DEHP结合，触发竞争性位移反应，释放适配体- dsdna探针。磁分离后，上清液通过Cas12a报告基因系统进行分析，Cas12a激活会裂解FAM-BHQ报告基因，产生荧光。与智能手机成像相结合，该方法可以快速获取结果。该传感器具有较高的选择性和灵敏度，DEHP的检测范围为1 pg/mL ~ 1 μg/mL，下限为0.15 pg/mL。有效检测各种真实样品中的DEHP，提供可靠的视觉监测。该方法为食品安全和环境污染监测提供了一种快速有效的现场检测策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.