A male sterility system with a novel bifunctional selection strategy for hybrid seed production in maize.

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-07-07 DOI:10.1111/nph.70372

Pan Yang,Yajie Sun,Ting Zheng,Chao Xu,Chaoyang Lin,Zhicheng Shen

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引用次数: 0

Abstract

Seed production technology (SPT), initially developed by DuPont Pioneer, provides a novel recessive genetic male sterility system for maize hybrid seed production. It is critical for this technology to safeguard the purity of male sterile seeds from contamination by transgenic maintainer seeds. Here, we report a modified seed production technology (mSPT), which utilizes nicosulfuron as a de-selection agent in addition to mechanical sorting based on the fluorescence of maintainer seeds to achieve close to 100% purity of male sterile plants for hybrid seed production. The maintainer line MAR46 for mSPT showed very low transgene transmission rates in multiple inbred backgrounds. Out of the 10 inbred lines, 7 of them were found to have non-transmission. The highest transmission rate was 0.035% for one inbred line. Importantly, the maintainer plants were found to be sensitive to nicosulfuron in different inbred backgrounds. Thus, the extra built-in decontamination technology in the mSPT system reported here was able to further safeguard the purity of sterile male plants by a convenient nicosulfuron spray to kill possible contaminated fertile transgenic maintainer plants.

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具有新型双功能选择策略的玉米雄性不育系统。

种子生产技术（SPT）是由杜邦先锋公司（DuPont Pioneer）率先开发的一种新型玉米杂交种子生产隐性遗传雄性不育系统。该技术的关键在于保证雄性不育种子的纯度不受转基因保持种子的污染。在这里，我们报道了一种改良制种技术（mSPT），该技术利用尼科磺隆作为去选择剂，并根据保持种子的荧光进行机械分选，以获得接近100%纯度的雄性不育植物用于杂交制种。mSPT保持系MAR46在多个近交系背景下表现出极低的转基因传输率。在10个自交系中，有7个被发现没有传播。一个自交系的最高传输率为0.035%。重要的是，在不同的近交系背景下，维持植株对镍磺隆敏感。因此，本文报道的mSPT系统中额外内置的去污技术能够通过方便的尼科磺隆喷雾杀死可能被污染的可育转基因维持植株，进一步保护不育雄性植株的纯度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.