Cross-sectional analysis of fibrosis-related gene expression in drug-induced gingival enlargement associated with periodontitis.

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-06-11 DOI:10.1016/j.oooo.2025.06.005

Jer-Haur Kuo, Li-Wen Lin

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Abstract

Objective: The objective of this study was to characterize the expression patterns of inflammation- and fibrosis-related genes in drug-induced gingival enlargement (DIGE) among patients with periodontitis.

Study design: Gingival tissue samples from 44 patients were categorized into 4 groups: N (healthy controls), P (periodontitis), A (patients with periodontitis receiving amlodipine and valsartan without DIGE), and G (periodontitis patients with DIGE). All specimens were obtained during periodontal flap surgery at least 6 months following initial therapy. Histomorphometric analysis, immunohistochemistry, and immunofluorescence staining were used to assess protein expression levels.

Results: Following initial therapy, no patient exhibited DIGE scores of 2 or 3. The expression levels of inflammation- and fibrosis-related markers, including cluster of differentiation 68, matrix metalloproteinase 12, a disintegrin and metalloproteinase 17, connective tissue growth factor, and cathepsin L, were elevated in group G compared with the other groups. Notably, group A demonstrated the highest expression of sirtuin 1 (SIRT1).

Conclusions: Effective supragingival and subgingival plaque control remains essential for managing DIGE in patients with periodontitis. The observed upregulation of SIRT1 in non-DIGE cases might indicate a potential antifibrotic role for this molecule.

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牙周炎相关的药物性牙龈扩大中纤维相关基因表达的横断面分析。

目的：本研究的目的是表征牙周炎患者药物性牙龈扩大（DIGE）中炎症和纤维化相关基因的表达模式。研究设计：将44例患者的牙龈组织样本分为4组：N组（健康对照组）、P组（牙周炎组）、A组（接受氨氯地平和缬沙坦治疗但不使用DIGE的牙周炎患者）和G组（牙周炎患者同时使用DIGE）。所有标本均在初始治疗后至少6个月的牙周皮瓣手术期间获得。组织形态学分析、免疫组织化学和免疫荧光染色用于评估蛋白表达水平。结果：在初始治疗后，没有患者表现出DIGE评分为2或3。与其他各组相比，G组炎症和纤维化相关标志物的表达水平升高，包括分化簇68、基质金属蛋白酶12、崩解素和金属蛋白酶17、结缔组织生长因子和组织蛋白酶L。值得注意的是，A组sirtuin 1 （SIRT1）的表达最高。结论：有效的龈上和龈下菌斑控制对于治疗牙周炎患者的DIGE至关重要。在非dige病例中观察到的SIRT1上调可能表明该分子具有潜在的抗纤维化作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.