Aldehyde Dehydrogenase 6 Family Member A1 Facilitates Ti Implant Osseointegration in Hyperlipidemia.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-07-04 DOI:10.1111/odi.15342

Ya-Nan Wang, Lu Yu, Yingxue Liu, Xin Xu, Dongjiao Zhang, Shiyue Liu, Shaohua Ge

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引用次数: 0

Abstract

Objective: Hyperlipidemia seriously impairs titanium (Ti) implant osseointegration, and endogenous therapeutic targets remain elusive. Aldehyde dehydrogenase 6 family member A1 (ALDH6A1) plays an important role in redox regulation and lipid metabolism. However, its role in osseointegration under hyperlipidemia conditions has not been thoroughly investigated. This study aimed to elucidate the potential role of ALDH6A1 in implant osseointegration in hyperlipidemia mice.

Materials and methods: ALDH6A1 expression levels were detected in peri-implant tissue and in primary osteoblasts by qRT-PCR and western blot. The role of ALDH6A1 in reducing oxidative damage and improving osseointegration on Ti implant surface was evaluated by locally overexpressing ALDH6A1 with a lentiviral vector in peri-implant tissue in hyperlipidemia mice.

Results: ALDH6A1 expression significantly decreased in hyperlipidemia mice, compared with normal mice (p < 0.05). ALDH6A1 was downregulated in a high-fat environment, and silencing ALDH6A1 led to osteogenic dysfunction in osteoblasts. ALDH6A1 overexpression maintained redox homeostasis and osteogenic activity of primary osteoblasts and facilitated osseointegration in hyperlipidemia mice by reducing oxidative damage in peri-implant tissue.

Conclusion: ALDH6A1 is a key protein for facilitating osseointegration by alleviating reactive oxygen species levels in hyperlipidemia, and developing specific ALDH6A1 agonists would be a promising strategy for ameliorating osseointegration in hyperlipidemia patients.

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醛脱氢酶6家族成员A1促进Ti植入物在高脂血症中的骨整合。

目的：高脂血症严重损害钛（Ti）种植体的骨整合，其内源性治疗靶点尚不明确。醛脱氢酶6家族成员A1 （ALDH6A1）在氧化还原调控和脂质代谢中起重要作用。然而，其在高脂血症条件下骨整合中的作用尚未得到彻底的研究。本研究旨在阐明ALDH6A1在高脂血症小鼠种植体骨整合中的潜在作用。材料与方法：采用qRT-PCR和western blot检测种植体周围组织和原代成骨细胞中ALDH6A1的表达水平。通过慢病毒载体在高脂血症小鼠种植体周围组织中局部过表达ALDH6A1，评估ALDH6A1在减少钛种植体表面氧化损伤和促进骨整合中的作用。结果：与正常小鼠相比，ALDH6A1在高脂血症小鼠中的表达明显降低(p)。结论：ALDH6A1是通过降低高脂血症患者活性氧水平来促进骨整合的关键蛋白，开发特异性的ALDH6A1激动剂将是改善高脂血症患者骨整合的一种有希望的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.