Biological activities of optimized biosynthesized selenium nanoparticles using Proteus mirabilis PQ350419 alone or combined with chitosan and ampicillin against common multidrug-resistant bacteria.

IF 4.9 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Laila A Elshikiby, Zakaria A M Baka, Mohamed M El-Zahed
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引用次数: 0

Abstract

Background: One of the most common issues in the world is bacterial resistance and biofilms, which can prolong the healing period and the need for self-medication. Additionally, they may be linked to unsuccessful therapies, which raises death rates, healthcare expenses, and the need for additional hospitalization. Therefore, to protect the environment and improve human health, there is a need for the creative synthesis of novel antibacterial materials. Proteus mirabilis strain PQ350419 was isolated, identified, and utilized as an efficient bio-nano-factory for biosynthesizing selenium nanoparticles (Se NPs) and optimizing procedures. This study showcases a simple and cost-effective approach for green-synthesizing a selenium/chitosan/ampicillin nanocomposite (Se/CS/AMP) as a novel antibacterial and antibiofilm agent. Several analyses, such as transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, zeta analysis, and ultraviolet-visible (UV-Vis) spectroscopy, were utilized to confirm and characterize the production of Se NPs and Se/CS/AMP. The absorption peaks for Se NPs and Se/CS/AMP were identified to be between 350 and 360 nm. The XRD data revealed the crystalline composition of the Se NPs loaded with CS and AMP. The FTIR spectra confirmed the presence of proteins that act as supporting and binding agents during synthesis. The stability of the prepared nanomaterials is improved by a strong negative surface charge of - 24.27 mV for Se NPs and - 23.92 mV for Se/CS/AMP. The particle sizes of Se NPs and Se/CS/AMP are shown by TEM to be in the ranges of 88-98 nm and 86-129 nm, respectively. Se NPs, either alone or in combination with chitosan (CS) and ampicillin (AMP), exhibited strong antibacterial activity against methicillin-resistant Staphylococcus aureus ATCC 43,300, Bacillus cereus ATCC 14,579, Klebsiella pneumoniae ATCC 11,296, and P. mirabilis PQ350419 in a dose-dependent manner. Compared to Se NPs and the common antibiotic AMP, the Se/CS/AMP combination demonstrated superior antibacterial activity. In comparison to Se NPs (40, 70, 110, and 150 µg/ml, respectively), the nanocomposite produced MIC values of 30, 40, 60, and 100 µg/ml against B. cereus, S. aureus, K. pneumoniae, and P. mirabilis. When compared to untreated cells, treated cells exhibited significant morphological changes and deformities, such as cell wall distortion, the separation of the cell wall from the plasma membrane, the formation of vacuoles, and complete cell lysis, according to TEM ultrastructure studies of bacteria treated with nanocomposite. Se/CS/AMP at 100 µg/ml was sufficient to prevent biofilm formation by up to 50% in S. aureus, K. pneumoniae, and P. mirabilis. The cell viability of the Vero cell line was significantly reduced (p˂0.05) in the cytotoxicity test of Se NPs alone at a concentration of 40.95 ± 2.34 µg/ml, and in its nanocomposite at a concentration of 199.09 ± 2.61 µg/ml. This indicates the nanocomposite's safety by showing its minimal harmful impact on the Vero cell line.

Conclusion: Se/CS/AMP has revealed an antibacterial and antibiofilm agent that could be useful in various industrial, medicinal, and environmental applications. This study introduces a work that presents an alternative, safe, promising, and efficient nanocomposite for treating harmful bacteria in humans and animals. This treatment is based on the synergistic effectiveness of Se NPs, CS, and AMP.

奇异变形杆菌PQ350419单独或与壳聚糖、氨苄西林联合合成的硒纳米粒子对常见多重耐药菌的生物活性研究。
背景:世界上最常见的问题之一是细菌耐药性和生物膜,这可以延长愈合时间和需要自我药物治疗。此外,它们可能与不成功的治疗有关,这增加了死亡率、医疗费用和额外住院的需要。因此,为了保护环境,改善人类健康,需要创造性地合成新型抗菌材料。分离鉴定了奇异变形杆菌PQ350419,并将其作为生物合成硒纳米粒子(Se NPs)的高效生物纳米工厂。本研究展示了一种简单、经济的绿色合成硒/壳聚糖/氨苄西林纳米复合材料(Se/CS/AMP)作为新型抗菌和抗生物膜剂的方法。利用透射电镜(TEM)、x射线衍射(XRD)、傅里叶变换红外(FTIR)光谱、zeta分析和紫外可见(UV-Vis)光谱等多种分析方法,对Se NPs和Se/CS/AMP的生产进行了确认和表征。Se NPs和Se/CS/AMP的吸收峰在350 ~ 360 nm之间。XRD数据显示了负载CS和AMP的Se NPs的晶体组成,FTIR光谱证实了合成过程中作为支撑和结合剂的蛋白质的存在。Se NPs和Se/CS/AMP的表面负电荷分别为- 24.27 mV和- 23.92 mV,提高了纳米材料的稳定性。TEM显示Se NPs和Se/CS/AMP的粒径分别在88 ~ 98 nm和86 ~ 129 nm之间。Se NPs单独或与壳聚糖(CS)和氨苄西林(AMP)联合对耐甲氧西林金黄色葡萄球菌(ATCC) 43,300、蜡样芽孢杆菌(ATCC) 14,579、肺炎克雷伯菌(ATCC) 11,296和P. mirabilis PQ350419均表现出较强的抑菌活性,且呈剂量依赖性。与Se NPs和常用抗生素AMP相比,Se/CS/AMP组合具有更强的抗菌活性。与Se NPs(分别为40、70、110和150µg/ml)相比,纳米复合材料对蜡样芽孢杆菌、金黄色葡萄球菌、肺炎克雷伯菌和神奇假单胞菌的MIC值分别为30、40、60和100µg/ml。根据纳米复合材料处理细菌的TEM超微结构研究,与未处理的细胞相比,处理过的细胞表现出明显的形态变化和畸形,如细胞壁扭曲、细胞壁与质膜分离、液泡形成和完全的细胞裂解。100µg/ml的Se/CS/AMP足以阻止金黄色葡萄球菌、肺炎克雷伯菌和神奇假单胞菌中高达50%的生物膜形成。在浓度为40.95±2.34µg/ml的Se NPs和浓度为199.09±2.61µg/ml的Se NPs的细胞毒性试验中,Vero细胞系的细胞活力显著降低(p小于0.05)。这表明纳米复合材料的安全性,显示其对Vero细胞系的有害影响最小。结论:Se/CS/AMP是一种广泛应用于工业、医药和环境等领域的抗菌和抗生物膜剂。本研究介绍了一种替代的、安全的、有前途的、有效的纳米复合材料,用于治疗人类和动物体内的有害细菌。这种治疗是基于Se NPs、CS和AMP的协同效果。
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来源期刊
Microbial Cell Factories
Microbial Cell Factories 工程技术-生物工程与应用微生物
CiteScore
9.30
自引率
4.70%
发文量
235
审稿时长
2.3 months
期刊介绍: Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems
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