Isolation and characterization of a novel glycoside from the inner bark of sclerocarya birrea: insights into antibiotic and anticancer activity.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-07-05 DOI:10.1080/14786419.2025.2524608

Mokgadi Precious Mphahlele, Armand Mallé Lando, Michael Hermann Kamdem Kengne, Jordan Tonga Lembe, Pangaman Jiyane, Thierry Fonkui Youmbi, Vusani Mandiwana, Lerato Hlekelele, Marthe Carine Djuidje Fotsing, Edwin Mpho Mmutlane, Stephani Zingue, Derek Tantoh Ndinteh

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引用次数: 0

Abstract

Phytochemical analysis of the dichloromethane-methanol (1:1) extract from Sclerocarya birrea inner bark led to the identification of three compounds: a novel glycoside, 3α-sorbithoxyglucose (1), and two known compounds, retusasterol (2) and β-sitosterol-3-O-D-glycoside (3), isolated for the first time from this plant. Structural elucidation via NMR confirmed their identities. Cytotoxicity studies against MCF7 breast cancer and DU145, PC3, LNCaP prostate cancer cell lines revealed that compounds 1 and 2 lacked inhibitory effects on cell proliferation, while retusasterol exhibited cytotoxicity with CC₅₀ values of 38 μg/mL (DU145), 40 μg/mL (PC3), and 32 μg/mL (LNCaP). However, further analysis indicated that retusasterol promoted tumour cell proliferation. Antimicrobial screening of 3α-sorbithoxyglucose against Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Mycobacterium smegmatis, Enterobacter cloacae, Proteus vulgaris, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, and Pseudomonas aeruginosa revealed MIC values of 15.625-250 μg/mL, suggesting potential as an antimicrobial agent compared to standard antibiotics.

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从硬核树皮中分离和表征一种新的糖苷：抗生素和抗癌活性的见解。

通过对birrea硬核栎内树皮二氯甲烷-甲醇（1:1）提取物的植物化学分析，鉴定出三种化合物：一种新的糖苷，3α-山梨羟基葡萄糖(1)，以及两种首次从该植物中分离到的已知化合物，reusasterol(2)和β-谷甾醇-3- o - d -糖苷(3)。核磁共振结构鉴定证实了它们的身份。对MCF7乳腺癌和DU145、PC3、LNCaP前列腺癌细胞的细胞毒性研究表明，化合物1和2对细胞增殖无抑制作用，而红芪甾醇的细胞毒性CC50值分别为38 μg/mL （DU145）、40 μg/mL （PC3）和32 μg/mL （LNCaP）。然而，进一步的分析表明，红蜡黄醇促进肿瘤细胞增殖。3α-山梨氧葡萄糖对枯草芽孢杆菌、粪肠球菌、表皮葡萄球菌、金黄色葡萄球菌、耻垢分枝杆菌、阴沟肠杆菌、普通变形杆菌、氧化克雷伯菌、肺炎克雷伯菌、神奇变形杆菌、大肠杆菌和铜绿假单胞菌的MIC值为15.625 ~ 250 μg/mL，与标准抗生素相比具有潜在的抗菌潜力。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.