{"title":"Isolation and characterization of a novel glycoside from the inner bark of <i>sclerocarya birrea</i>: insights into antibiotic and anticancer activity.","authors":"Mokgadi Precious Mphahlele, Armand Mallé Lando, Michael Hermann Kamdem Kengne, Jordan Tonga Lembe, Pangaman Jiyane, Thierry Fonkui Youmbi, Vusani Mandiwana, Lerato Hlekelele, Marthe Carine Djuidje Fotsing, Edwin Mpho Mmutlane, Stephani Zingue, Derek Tantoh Ndinteh","doi":"10.1080/14786419.2025.2524608","DOIUrl":null,"url":null,"abstract":"<p><p>Phytochemical analysis of the dichloromethane-methanol (1:1) extract from <i>Sclerocarya birrea</i> inner bark led to the identification of three compounds: a novel glycoside, 3α-sorbithoxyglucose (<b>1</b>), and two known compounds, retusasterol (<b>2</b>) and <i>β</i>-sitosterol-3-<i>O-D-</i>glycoside (<b>3</b>), isolated for the first time from this plant. Structural elucidation <i>via</i> NMR confirmed their identities. Cytotoxicity studies against MCF7 breast cancer and DU145, PC3, LNCaP prostate cancer cell lines revealed that compounds <b>1</b> and <b>2</b> lacked inhibitory effects on cell proliferation, while retusasterol exhibited cytotoxicity with CC<sub>50</sub> values of 38 μg/mL (DU145), 40 μg/mL (PC3), and 32 μg/mL (LNCaP). However, further analysis indicated that retusasterol promoted tumour cell proliferation. Antimicrobial screening of 3α-sorbithoxyglucose against <i>Bacillus subtilis</i>, <i>Enterococcus faecalis</i>, <i>Staphylococcus epidermidis</i>, <i>Staphylococcus aureus</i>, <i>Mycobacterium smegmatis</i>, <i>Enterobacter cloacae</i>, <i>Proteus vulgaris</i>, <i>Klebsiella oxytoca</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i> revealed MIC values of 15.625-250 μg/mL, suggesting potential as an antimicrobial agent compared to standard antibiotics.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.9000,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2025.2524608","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Phytochemical analysis of the dichloromethane-methanol (1:1) extract from Sclerocarya birrea inner bark led to the identification of three compounds: a novel glycoside, 3α-sorbithoxyglucose (1), and two known compounds, retusasterol (2) and β-sitosterol-3-O-D-glycoside (3), isolated for the first time from this plant. Structural elucidation via NMR confirmed their identities. Cytotoxicity studies against MCF7 breast cancer and DU145, PC3, LNCaP prostate cancer cell lines revealed that compounds 1 and 2 lacked inhibitory effects on cell proliferation, while retusasterol exhibited cytotoxicity with CC50 values of 38 μg/mL (DU145), 40 μg/mL (PC3), and 32 μg/mL (LNCaP). However, further analysis indicated that retusasterol promoted tumour cell proliferation. Antimicrobial screening of 3α-sorbithoxyglucose against Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Mycobacterium smegmatis, Enterobacter cloacae, Proteus vulgaris, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, and Pseudomonas aeruginosa revealed MIC values of 15.625-250 μg/mL, suggesting potential as an antimicrobial agent compared to standard antibiotics.
期刊介绍:
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.