Evaluation of humoral immunity and maternal antibody transfer in Nile tilapia (Oreochromis niloticus) broodstock following immunization with a bivalent vaccine

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-07-05 DOI:10.1016/j.fsi.2025.110545

Andrea N. Pardo , Ha Thanh Dong , Krishna R. Salin , Saengchan Senapin

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引用次数: 0

Abstract

Aeromonas veronii and Streptococcus agalactiae are significant bacterial pathogens threatening Nile tilapia (Oreochromis niloticus) aquaculture globally. Coinfections with these pathogens are prevalent in aquaculture environments, underscoring the need for the application of a bivalent vaccine. This study investigated the humoral immune response kinetics of Nile tilapia broodstock post vaccination, examined maternal antibody (immunoglobulin M-IgM) transfer to their offspring and assessed the vaccine's protective efficacy through a passive immunization and pathogen challenges. Broodstock were immunized with an optimized bivalent vaccine, followed by two booster injections, and serum IgM levels were monitored using the enzyme-linked immunosorbent assay (ELISA). The results revealed that the vaccinated fish elicited specific IgM against both pathogens, with higher levels of anti-A. veronii antibodies compared to anti-S. agalactiae antibodies following primary and booster doses. The detection of specific IgM in fertilized eggs indicated the transfer of maternal immunity. However, the decline of these antibodies to undetectable levels in fry following yolk sac absorption indicated a transient persistence of the transferred antibodies. In the passive immunization study, fish administered sera from vaccinated broodstock demonstrated relative percent survival (RPS) of 35.7 % and 83.3 % against A. veronii and S. agalactiae, respectively. These results implied the substantial contribution of humoral immunity in defense against both pathogens. In conclusion, the findings of this study provided valuable insights into the humoral immune response in tilapia broodstock and confirmed maternal immunity transfer following bivalent vaccination. Broodstock vaccination may therefore serve as a promising biosecurity strategy to prevent infection in broodstock and enhance fry immunity, warranting further investigation.

查看原文本刊更多论文

尼罗罗非鱼（Oreochromis niloticus）亲鱼接种二价疫苗后体液免疫和母源抗体转移的评价。

维罗氏气单胞菌和无乳链球菌是威胁尼罗罗非鱼养殖的重要致病菌。与这些病原体的共感染在水产养殖环境中很普遍，这强调了应用二价疫苗的必要性。本研究研究了尼罗罗非鱼接种疫苗后的体液免疫反应动力学，检测了母源抗体（免疫球蛋白M-IgM）向其后代的转移，并通过被动免疫和病原体挑战评估了疫苗的保护效果。用优化后的二价疫苗对雏鸡进行免疫，然后进行两次加强注射，并使用酶联免疫吸附试验（ELISA）监测血清IgM水平。结果显示，接种疫苗的鱼对这两种病原体都产生了特异性IgM，具有更高水平的抗a抗体。veronii抗体与抗s。初级和加强剂量后无乳抗体。受精卵中特异性IgM的检测表明母体免疫转移。然而，在卵黄囊吸收后，这些抗体在煎炸中下降到无法检测的水平，表明转移抗体的短暂持久性。在被动免疫研究中，接种过疫苗的亲鱼血清对维罗氏弧菌和无乳链球菌的相对存活率分别为35.7%和83.3%。这些结果暗示了体液免疫在防御这两种病原体方面的重要贡献。总之，本研究的发现为罗非鱼亲鱼的体液免疫反应提供了有价值的见解，并证实了二价疫苗接种后母体免疫转移。因此，种苗可作为一种有希望的生物安全策略，以预防种苗感染和增强种苗免疫力，值得进一步研究。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.