Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.

IF 2.2 3区医学 Q3 ENGINEERING, BIOMEDICAL

Artificial organs Pub Date : 2025-07-05 DOI:10.1111/aor.15048

Chuanbo Xu, Huajiang Liu, Jiantao She, Tao Xu, Yuan Zhou, Xuebo Yan, Tiangang Li, Bin Dai

{"title":"Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.","authors":"Chuanbo Xu, Huajiang Liu, Jiantao She, Tao Xu, Yuan Zhou, Xuebo Yan, Tiangang Li, Bin Dai","doi":"10.1111/aor.15048","DOIUrl":null,"url":null,"abstract":"Objective: This study aimed to determine the more suitable method for constructing parathyroid organoids.Methods: Parathyroid organoids were established using two different culture conditions, and organoid morphology and ATP activity were assessed to evaluate their performance. Organoid identity and function were confirmed by STR profiling, histology, immunofluorescence, and ELISA. Organoids were further treated with different calcium concentrations, and changes in morphology, ATP activity, intracellular calcium, CaSR expression, and PTH secretion were examined.Results: Organoids cultured in VEGF-121- and angiotensin-negative medium showed no noticeable morphological change from Day 1 to Day 3, and ATP activity declined over the same period. In contrast, organoids in VEGF-121+ and angiotensin-positive medium exhibited increased number and ATP activity over the same period. These organoids shared the same mutation profile and structural features as the original tissues and expressed PTH, GCM2, CaSR, and CHGA. PTH secretion remained stable across passages. Low calcium had minimal impact on morphology, viability, and PTH release but reduced intracellular calcium levels and CaSR expression. High calcium promoted ATP activity and elevated intracellular calcium, while suppressing PTH secretion and upregulating CaSR expression.Conclusions: VEGF-121+ and angiotensin-positive medium better supported the generation and maintenance of functional parathyroid organoids. This provides an in vitro model for further investigation into parathyroid-related signaling and applications in regenerative medicine.","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial organs","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1111/aor.15048","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This study aimed to determine the more suitable method for constructing parathyroid organoids.

Methods: Parathyroid organoids were established using two different culture conditions, and organoid morphology and ATP activity were assessed to evaluate their performance. Organoid identity and function were confirmed by STR profiling, histology, immunofluorescence, and ELISA. Organoids were further treated with different calcium concentrations, and changes in morphology, ATP activity, intracellular calcium, CaSR expression, and PTH secretion were examined.

Results: Organoids cultured in VEGF-121- and angiotensin-negative medium showed no noticeable morphological change from Day 1 to Day 3, and ATP activity declined over the same period. In contrast, organoids in VEGF-121+ and angiotensin-positive medium exhibited increased number and ATP activity over the same period. These organoids shared the same mutation profile and structural features as the original tissues and expressed PTH, GCM2, CaSR, and CHGA. PTH secretion remained stable across passages. Low calcium had minimal impact on morphology, viability, and PTH release but reduced intracellular calcium levels and CaSR expression. High calcium promoted ATP activity and elevated intracellular calcium, while suppressing PTH secretion and upregulating CaSR expression.

Conclusions: VEGF-121+ and angiotensin-positive medium better supported the generation and maintenance of functional parathyroid organoids. This provides an in vitro model for further investigation into parathyroid-related signaling and applications in regenerative medicine.

查看原文本刊更多论文

甲状旁腺类器官构建方法的比较及钙对类器官功能的影响。

目的：探讨构建甲状旁腺类器官的合适方法。方法：在两种不同的培养条件下建立甲状旁腺类器官，并对类器官形态和ATP活性进行评价。通过STR分析、组织学、免疫荧光和ELISA证实了类器官的身份和功能。进一步用不同钙浓度处理类器官，观察其形态学、ATP活性、细胞内钙、CaSR表达和甲状旁腺激素分泌的变化。结果：在VEGF-121和血管紧张素阴性培养基中培养的类器官从第1天到第3天没有明显的形态学变化，ATP活性在同一时期下降。相比之下，VEGF-121+和血管紧张素阳性培养基中的类器官在同一时期表现出增加的数量和ATP活性。这些类器官与原始组织具有相同的突变谱和结构特征，表达PTH、GCM2、CaSR和CHGA。甲状旁腺激素分泌保持稳定。低钙对形态、活力和甲状旁腺激素释放的影响最小，但降低了细胞内钙水平和CaSR表达。高钙促进ATP活性和细胞内钙升高，抑制PTH分泌，上调CaSR表达。结论：VEGF-121+和血管紧张素阳性培养基能更好地支持功能性甲状旁腺类器官的生成和维持。这为进一步研究甲状旁腺相关信号传导及其在再生医学中的应用提供了体外模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Artificial organs 工程技术-工程：生物医学

CiteScore

4.30

自引率

12.50%

发文量

303

审稿时长

4-8 weeks

期刊介绍： Artificial Organs is the official peer reviewed journal of The International Federation for Artificial Organs (Members of the Federation are: The American Society for Artificial Internal Organs, The European Society for Artificial Organs, and The Japanese Society for Artificial Organs), The International Faculty for Artificial Organs, the International Society for Rotary Blood Pumps, The International Society for Pediatric Mechanical Cardiopulmonary Support, and the Vienna International Workshop on Functional Electrical Stimulation. Artificial Organs publishes original research articles dealing with developments in artificial organs applications and treatment modalities and their clinical applications worldwide. Membership in the Societies listed above is not a prerequisite for publication. Articles are published without charge to the author except for color figures and excess page charges as noted.