The role of sodium pyruvate in mitigating the cytotoxic effects of vanadium on CHO-K1 cells.

IF 3.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Iwona Zwolak, Ewa Wnuk, Elżbieta Kochanowicz
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Abstract

Vanadium is a hazardous, pro-oxidant element that contributes to environmental pollution and has been reported as a risk factor for human health through occupational or environmental exposure. Pyruvate, on the other hand, is a natural alpha-keto acid with exceptional antioxidant and cytoprotective properties. Therefore, the aim of this study was to evaluate the mitigating effect of exogenous pyruvate against vanadium-induced toxicity in cultured Chinese hamster ovary (CHO)-K1 cells. To this end, CHO-K1 cells were exposed to 100 μM vanadyl sulfate (VOSO4) for 24 h in the presence of 4.5 and 8 mM sodium pyruvate. Cell proliferation and morphological changes, cellular ATP levels, antioxidant stress (GSH) levels and apoptosis markers (caspase 3, 9, annexin V binding) were assessed to investigate the effect of sodium pyruvate on VOSO4-induced damage in CHO-K1 cells. The results showed that VOSO4 induced morphological changes, inhibited cell proliferation, decreased cellular ATP and reduced glutathione levels. Co-treatment of VOSO4-intoxicated CHO-K1 cells with sodium pyruvate significantly reduced these cytotoxic effects. Analysis of apoptosis and necrosis showed that VOSO4 slightly induced apoptosis and necrosis, and exogenous pyruvate inhibited the cytotoxicity of the tested vanadium dose in CHO-K1 cells, mainly by reducing the necrosis effect. The cytoprotective effect of exogenous pyruvate was also confirmed in normal mouse fibroblast (NIH/3T3) cells demonstrating that the protective properties of pyruvate are not cell specific.

丙酮酸钠在减轻钒对CHO-K1细胞毒性作用中的作用。
钒是一种有害的促氧化元素,会造成环境污染,据报道,由于职业或环境接触,钒是人类健康的一个风险因素。另一方面,丙酮酸是一种天然的α -酮酸,具有特殊的抗氧化和细胞保护特性。因此,本研究旨在评价外源性丙酮酸对钒诱导的中国仓鼠卵巢(CHO)-K1细胞毒性的缓解作用。为此,CHO-K1细胞在4.5和8 mM丙酮酸钠存在下暴露于100 μM硫酸钒(VOSO4)中24 h。通过观察丙酮酸钠对voso4诱导CHO-K1细胞损伤的影响,观察细胞增殖和形态学变化、细胞ATP水平、抗氧化应激(GSH)水平和凋亡标志物(caspase 3,9、膜联蛋白V结合)。结果表明,VOSO4诱导细胞形态改变,抑制细胞增殖,降低细胞ATP和谷胱甘肽水平。与丙酮酸钠共同处理voso4中毒的CHO-K1细胞可显著降低这些细胞毒性作用。凋亡和坏死分析显示,VOSO4轻微诱导CHO-K1细胞凋亡和坏死,外源性丙酮酸抑制了所试钒剂量对CHO-K1细胞的细胞毒性,主要是通过降低坏死作用。在正常小鼠成纤维细胞(NIH/3T3)中也证实了外源性丙酮酸的细胞保护作用,表明丙酮酸的保护特性不是细胞特异性的。
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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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