{"title":"Morphology engineering facilitates constructing efficient cell factories.","authors":"Ji-Yuan Sun, Xiao-Ran Jiang","doi":"10.1016/j.biotechadv.2025.108639","DOIUrl":null,"url":null,"abstract":"<p><p>The utilization of microbial cell factories for industrial chemical production from renewable feedstocks provides a promising strategy for achieving sustainable biomanufacturing. However, cellular morphology significantly affects the efficacy of microbial cells as production platforms. Morphology engineering aims to \"harnessing energy products through enlarged\" by reprogramming cellular architecture across multiple scales, thereby unlocking cellular potential to develop high-performance microbial cell factories. This review summarizes the mechanisms for maintaining cell morphology in rod-shaped bacteria and yeasts. Subsequently, we analyze current three main applications of morphology engineering in optimizing microbial cell factories. By increasing the length and width of short rod-shaped bacteria, the cell volume is increased to promote the accumulation of intracellular products. By reducing the size of the mycelial globules of actinomycetes, nutrient absorption is promoted to increase the yield of natural products. By increasing the area of yeast organelles and cell membranes, the yield of terpene products is enhanced. Furthermore, the current limitations of morphology engineering and its future development directions are proposed. This provides theoretical frameworks and technical references for advancing morphology engineering.</p>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":" ","pages":"108639"},"PeriodicalIF":12.1000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology advances","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.biotechadv.2025.108639","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The utilization of microbial cell factories for industrial chemical production from renewable feedstocks provides a promising strategy for achieving sustainable biomanufacturing. However, cellular morphology significantly affects the efficacy of microbial cells as production platforms. Morphology engineering aims to "harnessing energy products through enlarged" by reprogramming cellular architecture across multiple scales, thereby unlocking cellular potential to develop high-performance microbial cell factories. This review summarizes the mechanisms for maintaining cell morphology in rod-shaped bacteria and yeasts. Subsequently, we analyze current three main applications of morphology engineering in optimizing microbial cell factories. By increasing the length and width of short rod-shaped bacteria, the cell volume is increased to promote the accumulation of intracellular products. By reducing the size of the mycelial globules of actinomycetes, nutrient absorption is promoted to increase the yield of natural products. By increasing the area of yeast organelles and cell membranes, the yield of terpene products is enhanced. Furthermore, the current limitations of morphology engineering and its future development directions are proposed. This provides theoretical frameworks and technical references for advancing morphology engineering.
期刊介绍:
Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.