Identification and application of the reductase from Wickerhamomyces ciferrii for the degradation of patulin

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-07-05 DOI:10.1016/j.ijbiomac.2025.145656

Zhaoxia Liu , Binbin Ouyang , Junmei Ding , Wei Xu , Wanmeng Mu

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Abstract

Patulin (PAT) is a mycotoxin widely found in fruits, which has various toxicological effects and seriously affects the safety of fruits and related products. Since PAT is stable in nature, developing bio-enzymatic degradation methods has become the predominant focus of current research. This study cloned a potential reductase gene capable of PAT degradation from Wickerhamomyces ciferrii and characterized the recombinant enzyme properties in detail. The recombinant enzyme is not metal ion dependent, and exhibited the highest activity at pH 6.5 and 50 °C for PAT degradation. Notably, the enzyme could degrade a wide range of PAT content from 5 to 40 μg/mL at a low enzyme concentration (25 μg/mL) in the presence of cofactor NADPH. The docking study of the enzyme and PAT as well as the virtual mutagenesis indicated that residues of Ser141, Tyr157 and His186 are significant for the transformation of PAT in the presence of NADPH.

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柳条霉降解展霉素还原酶的鉴定及应用

棒曲霉素（PAT）是一种广泛存在于水果中的真菌毒素，具有多种毒理作用，严重影响水果及相关产品的安全。由于PAT性质稳定，开发生物酶降解方法已成为当前研究的重点。本研究从枸橼酸Wickerhamomyces ciferrii中克隆了一个能够降解PAT的潜在还原酶基因，并对重组酶的性质进行了详细的表征。重组酶不依赖金属离子，在pH 6.5和50°C条件下具有最高的降解活性。值得注意的是，在辅酶NADPH存在的情况下，在较低的酶浓度（25 μg/mL）下，该酶可以降解5 ~ 40 μg/mL的PAT含量。该酶与PAT的对接研究以及虚拟诱变表明，Ser141、Tyr157和His186残基在NADPH存在下对PAT的转化具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.