Stripe rust effector Pst9653 interferes with subcellular distributions of catalase TaCAT3 to facilitate susceptibility-involved ROS scavenging in wheat.

IF 8.1 1区 生物学 Q1 Agricultural and Biological Sciences
New Phytologist Pub Date : 2025-07-03 DOI:10.1111/nph.70341
Xiaobo Wei, Nian Liu, Weiling Yang, Wenjie Hou, Yuanfei Hou, Zhensheng Kang, Jie Liu
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引用次数: 0

Abstract

Plants resist pathogen invasion by triggering reactive oxygen species (ROS) burst. However, the molecular mechanism by which pathogens suppress ROS accumulation for successful infection remains to be elucidated. To address this concern, an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, was characterized. The function of Pst9653 during Pst infection was identified by RNA interference and overexpression in wheat. The interaction targets of Pst9653 were screened by yeast two-hybrid assays and further regulatory mode was also determined. The results showed that Pst9653, encoding a virulence effector, was significantly upregulated during Pst infection. Silencing of Pst9653 led to increased wheat resistance to stripe rust and restricted fungal development, whereas overexpressing Pst9653 greatly promoted Pst susceptibility in wheat. In addition, Pst9653 was shown to interact with wheat catalases TaCAT1 and TaCAT3. TaCAT3 was induced by Pst and negatively regulated wheat resistance to stripe rust. Co-expression of TaCAT3 and Pst9653 facilitates cytoplasmic enrichment of TaCAT3 in plants. Our findings reveal that Pst9653 is secreted and disrupts TaCAT3 import into peroxisomes to promote susceptibility-involved ROS scavenging in wheat. This study uncovers a novel regulation strategy to cope with excessive ROS accumulation under stress conditions.

小麦条锈病效应因子Pst9653干扰过氧化氢酶TaCAT3的亚细胞分布,促进敏感性相关的ROS清除。
植物通过触发活性氧(ROS)爆发来抵抗病原体的入侵。然而,病原体抑制ROS积累以成功感染的分子机制仍有待阐明。为了解决这一问题,对小麦条锈病病原小麦条锈病(Pst)的效应蛋白Pst9653进行了鉴定。通过RNA干扰和过表达鉴定了Pst9653在小麦Pst侵染过程中的功能。通过酵母双杂交实验筛选Pst9653的相互作用靶点,并确定进一步的调控模式。结果表明,编码毒力效应的Pst9653在Pst感染期间显著上调。沉默Pst9653可提高小麦对条锈病的抗性,抑制真菌的发育,而过表达Pst9653可显著提高小麦对条锈病的敏感性。此外,Pst9653被证明与小麦过氧化氢酶TaCAT1和TaCAT3相互作用。Pst诱导的TaCAT3负调控小麦对条锈病的抗性。TaCAT3和Pst9653的共表达促进了植物细胞质中TaCAT3的富集。我们的研究结果表明,在小麦中,Pst9653被分泌并破坏TaCAT3进入过氧化物酶体,以促进与敏感性相关的ROS清除。本研究揭示了一种新的调控策略来应对应激条件下ROS的过度积累。
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来源期刊
New Phytologist
New Phytologist PLANT SCIENCES-
CiteScore
17.60
自引率
5.30%
发文量
728
审稿时长
1 months
期刊介绍: New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.
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