Jéssica Tatiane Sauthier, Jéssica Barreto Dias, Cristiane de Sousa Ferreira, Brendo de Oliveira Nascimento Gomes, Ketlyn Araujo Fraga, Elisa Cavalcante Pereira, Bruna Mendonça da Silva, Letícia Ferreira Lima, Irving Martins da Silveira Gonçalves, Audrien Alves Andrade de Souza, Marília Alves Figueira de Melo, Alexandre Araujo Cunha Dos Santos, Beatriz de Lima Alessio Müller, Aline Dos Santos Moreira, Paola Cristina Resende, Eduardo de Mello Volotão, Edson Elias da Silva
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引用次数: 0
Abstract
Background: Human enterovirus C99 (HEV-C99) is a member of the species Enterovirus C. Currently, three complete genomes of HEV-C99 were reported in Brazil, all obtained from children with gastroenteritis symptoms. Notwithstanding, no HEV-C99 complete genome associated with AFP cases in Brazil have been analysed so far.
Objectives: In light of this, molecular characterisation of an HEV-C99 isolated from a case of acute flaccid paralysis (AFP) in Brazil was carried out.
Methods: In 2005, an HEV-C99 strain was isolated from a 2-year-old female child in Santa Catarina State, Brazil, showing classic symptoms of AFP. Stool sample was inoculated into specific cell cultures. Viral RNA was extracted, and polymerase chain reaction (PCR) were performed to amplify the VP1 gene; the sequence was analysed for molecular identification. Subsequently, the complete genome was sequenced and analysed, including a phylogenetic analysis of the VP1 gene.
Findings: The isolate, denominated HEV-C99/33322/BRA/2005 presented 85.85% identity to other HEV-C99 strains also described in Brazil, subsequently. Besides, the isolate grouped together with HEV-C99 cluster C strains. To our knowledge, this was the first described HEV-C99 isolated from an AFP case in Brazil.
Main conclusions: The data generated in this study bolster the role of HEV-C99 as an etiologic agent of AFP. Furthermore, this research enhances our knowledge regarding the HEV-C99 genetic diversity.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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