Antimicrobial resistance and virulence factors of Klebsiella quasipneumoniae, the novel sequence types (ST) 7979 and 7980 from Indonesia

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-07-01 DOI:10.1016/j.micpath.2025.107857

Putu Yuliandari , Ajeng Kusumaningtyas Pramono , Novaria Sari Dewi Panjaitan , Agriani Dini Pasiana , Dwi Tyastuti , Abdul Hadi Furqoni , Sarwo Handayani , Khariri Khariri , Fitriana Fitriana , Rita Marleta Dewi , Sunarno Sunarno , Nastiti Intan Permata Sari

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引用次数: 0

Abstract

Klebsiella pneumoniae is a human pathogen of global concern. The more recently described pathogen, K. quasipneumoniae, shares similar morphological characteristics with K. pneumoniae and is commonly misidentified as this species using conventional laboratory techniques. This study investigates the molecular characteristics of four phenotype-identified K. pneumoniae isolates obtained from hospital wastewater in Jakarta, Indonesia. Whole-genome sequencing (WGS) and the Average Nucleotide Identity (ANI) showed that these isolates were eventually identified as K. quasipneumoniae subsp. quasipneumoniae, a closely related species of K. pneumoniae. These isolates of novel ST7979 and ST7980 strains are classified as multi-drug resistant (MDR) bacteria and harbor many antibiotic-resistance genes. Interestingly, the novel ST7980 strain is carbapenem non-susceptible and harbors the sul1 gene and the heat-stable enterotoxin gene, astA. The ST7979 strains have KL55 capsular type and O3b type, whereas the ST7980 strains have KL107 and O12 types. Our finding highlights the significance of identifying the K. quasipneumoniae strain utilizing a genomic platform. Additionally, routine surveillance is needed to monitor the hospital wastewater and avoid the spread of multidrug-resistant bacteria.

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印尼拟肺炎克雷伯菌新序列型（ST） 7979和7980的耐药性和毒力因子分析

肺炎克雷伯菌是一种引起全球关注的人类病原体。最近描述的病原体，准肺炎克雷伯菌，与肺炎克雷伯菌具有相似的形态特征，使用常规实验室技术通常被误认为是该物种。本研究调查了从印度尼西亚雅加达医院废水中获得的四种表型鉴定的肺炎克雷伯菌分离株的分子特征。全基因组测序（WGS）和平均核苷酸鉴定（ANI）结果表明，这些分离株最终被鉴定为准肺炎克雷伯菌亚种。准肺炎菌，是肺炎克雷伯菌的一个近缘种。这些新分离的ST7979和ST7980菌株被归类为多重耐药（MDR）细菌，含有许多抗生素耐药基因。有趣的是，新的ST7980菌株对碳青霉烯不敏感，含有sul1基因和热稳定型肠毒素基因astA。ST7979株具有KL55荚膜型和O3b型，ST7980株具有KL107型和O12型。我们的发现强调了利用基因组平台鉴定准肺炎克雷伯菌菌株的重要性。此外，需要进行常规监测，对医院废水进行监测，避免耐多药细菌的传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)