Using Digested and Lyophilized Bone Matrices to Enhance Retention and Sustained Release of BMP-2 for Bone Regeneration.

IF 1 4区 医学 Q3 SURGERY
Austin Stellpflug, Sameer Shakir, Kaleb Keener, Christopher Nguyen, Milan Patel, Kant Lin, Bo Wang
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引用次数: 0

Abstract

Background: Human recombinant bone morphogenetic protein 2 (rhBMP-2) is an FDA-approved growth factor that is shown to induce new bone formation but includes safety risks like inflammation and ectopic bone formation. Research indicates that a prolonged, controlled release of rhBMP-2 enhances bone healing with fewer side effects than short-term release. Collagen sponges, commonly used for this purpose, have drawbacks including poor structural stability and inadequate release profiles. Current research is focused on improving rhBMP-2 delivery strategies, particularly for pediatric patients where its use is considered off-label.

Methods: The authors' previous study developed a sponge-like gellable bone matrix from decellularized, demineralized, and enzymatically digested porcine bone that demonstrated both biocompatibility and bone regenerative capabilities. This study expands our previous work by infusing synthetic BMP2 (sBMP2) into digested and lyophilized bone matrix (DLBM) and stabilizing it with glutaraldehyde (GL) crosslinking, referred to as DLBM/sBMP2/GL scaffold. The authors evaluate the release of sBMP2 in vitro from within the DLBM post stabilization, and the overall effectiveness of the DLBM/sBMP2/GL scaffold at stimulating bone growth in vivo using a nonhealing, surgically created tibial defect in a small animal model.

Results: The DLBM/sBMP2/GL scaffold offers a prolonged and sustained release of sBMP2 in vitro and demonstrates biocompatibility and osteogenicity in vivo, resulting in substantial bone regeneration based on the gradual scaffold degradation with the sustained release of sBMP2.

Conclusions: The resulting DLBM/sBMP2/GL scaffold provides a promising alternative grafting option for pediatric patients, offering improved safety and efficacy over existing methods. This innovative approach addresses the need for better rhBMP-2 delivery systems, potentially expanding its use in pediatric bone healing applications.

利用消化和冻干骨基质增强BMP-2的保留和持续释放,促进骨再生。
背景:人重组骨形态发生蛋白2 (rhBMP-2)是fda批准的一种生长因子,可诱导新骨形成,但存在炎症和异位骨形成等安全风险。研究表明,长期、有控制地释放rhBMP-2能促进骨愈合,而且副作用比短期释放少。胶原蛋白海绵,通常用于此目的,有缺点,包括结构稳定性差和不充分的释放轮廓。目前的研究重点是改进rhBMP-2的分娩策略,特别是对于儿科患者,其使用被认为是标签外的。方法:作者先前的研究从脱细胞、脱矿和酶消化的猪骨中开发了海绵状凝胶状骨基质,证明了生物相容性和骨再生能力。本研究扩展了我们之前的工作,将合成的BMP2 (sBMP2)注入消化和冻干的骨基质(DLBM)中,并用戊二醛(GL)交联稳定,称为DLBM/sBMP2/GL支架。作者评估了稳定后DLBM内sBMP2的体外释放,以及DLBM/sBMP2/GL支架在小动物模型中使用未愈合的手术造成的胫骨缺损刺激体内骨生长的总体有效性。结果:DLBM/sBMP2/GL支架在体外具有sBMP2的延长和持续释放,在体内具有生物相容性和成骨性,在sBMP2持续释放的基础上,支架逐渐降解,实现了大量的骨再生。结论:所得到的DLBM/sBMP2/GL支架为儿科患者提供了一种有希望的替代移植选择,比现有方法具有更高的安全性和有效性。这种创新的方法解决了对更好的rhBMP-2递送系统的需求,潜在地扩大了其在儿科骨愈合应用中的应用。
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来源期刊
CiteScore
1.70
自引率
11.10%
发文量
968
审稿时长
1.5 months
期刊介绍: ​The Journal of Craniofacial Surgery serves as a forum of communication for all those involved in craniofacial surgery, maxillofacial surgery and pediatric plastic surgery. Coverage ranges from practical aspects of craniofacial surgery to the basic science that underlies surgical practice. The journal publishes original articles, scientific reviews, editorials and invited commentary, abstracts and selected articles from international journals, and occasional international bibliographies in craniofacial surgery.
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