Exosomes carrying lncRNA FLG-AS1 overexpression vectors inhibit the tumorigenesis of oral squamous cell carcinoma via fat mass and obesity-associated protein-mediated m6A modification.
IF 2 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0
Abstract
Long noncoding RNAs (lncRNAs) selectively incorporated into exosomes contribute to the tumorigenesis of various cancers. However, the role of the lncRNA FLG-AS1 in oral squamous cell carcinoma (OSCC) has yet to be investigated. Hence, the current study aimed to evaluate the potential role of exosome-derived FLG-AS1 in OSCC. The expression of FLG-AS1 and fat mass and obesity-associated protein (FTO) in OSCC samples were determined via real-time quantitative polymerase chain reaction (RT-qPCR). The role of FLG-AS1 in OSCC was investigated by performing CCK8, flow cytometry, and transwell assays. Additionally, bioinformatics, methylated (m6A) RNA immunoprecipitation, and RT-qPCR were performed to evaluate the n6-methyladenosine (m6A) modification of FLG-AS1 and its underlying mechanism. Characterization of exosomal FLG-AS1 in OSCC cells was achieved through transmission electron microscopy, nanoparticle tracking analysis, Western blot analysis of CD63 and CD9, and RT-qPCR. Finally, the effects of exosomal FLG-AS1 on OSCC cell malignancy was analyzed using the aforementioned cell-based assays. Overall, our findings showed that low FLG-AS1 expression in OSCC restricted the malignant properties of OSCC cells. Furthermore, FTO reduced FLG-AS1 expression by mediating its m6A demethylation in OSCC cells. Exosomal FLG-AS1 expression was upregulated in OSCC cells, suggesting that it was packaged in the OSCC cell-derived exosomes, whereas exosome-derived FLG-AS1 overexpression vector reduced the malignant potential of the target OSCC cells. In conclusion, exosomal FLG-AS1 functions as a tumor suppressor in OSCC, while FTO can impair FLG-AS1 expression through m6A demethylation.
期刊介绍：
The scope of the Journal includes:
1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products.
2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools.
3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research.
4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy.
5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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