Transcriptome analysis of granulosa cell layer of hierarchical follicles during the egg-laying interval in pigeons.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2025-07-04 DOI:10.1080/00071668.2025.2518608

J Chen, Y Wang, X Wu, C Zhang, D Miao, Y Wang, H Yang, Z Wang

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引用次数: 0

Abstract

1. Follicular development involves a series of critical processes, including the recruitment and selection of follicles, regulated by complex mechanisms. However, the specific genes and molecular mechanisms involved in follicular development in pigeons during the egg-laying interval (LI) are still unknown.2. To identify key genes that regulate follicular development in pigeons, the largest follicles (F1) and the second-largest follicles (F2) were collected on the third (LI3), fifth (LI5) and seventh (LI7) days of the LI for RNA sequencing and bioinformatics analyses.3. In F1 follicle granulosa cells (F1GC), 7,743 and 2,791 differentially expressed genes (DEG) were identified in the LI3 vs. LI5 group and the LI5 vs. LI7 group, respectively. In F2 follicle granulosa cells (F2GC), 3,072 DEG were detected in the LI5 vs. LI7 group.4. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analyses were performed and a protein-protein network for the DEG was constructed. This allowed the identification of hub genes (FKBP4, RAF1, CTNNB1, ENO1, FST and PHB) associated with hierarchical follicular development in pigeons.

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鸽子产蛋期分层卵泡颗粒细胞层转录组分析。

1. 卵泡发育涉及一系列关键过程，包括卵泡的募集和选择，受复杂机制的调控。然而，鸽子产蛋期卵泡发育的具体基因和分子机制尚不清楚。为了确定调控鸽子卵泡发育的关键基因，在第3天（LI3）、第5天（LI5）和第7天（LI7）采集最大卵泡（F1）和第2卵泡（F2）进行RNA测序和生物信息学分析。在F1卵泡颗粒细胞（F1GC）中，LI3与LI5组和LI5与LI7组分别鉴定出7,743和2,791个差异表达基因（DEG）。在F2卵泡颗粒细胞（F2GC）中，LI5组和LI7组检测到3072℃。对基因本体（GO）和京都基因与基因组百科全书（KEGG）进行了分析，并构建了基因本体的蛋白质-蛋白质网络。这使得鉴定出与鸽子分层卵泡发育相关的中心基因（FKBP4、RAF1、CTNNB1、ENO1、FST和PHB）成为可能。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .