The impact of initial cooling rates on cell preservation in frozen water-dimethyl sulfoxide media: a morphological study.

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2025-07-04 DOI:10.1007/s44211-025-00815-8

Rinko Sabanai, Yoshifumi Suzuki, Takafumi Mizushige, Nobuo Uehara, Arinori Inagawa

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引用次数: 0

Abstract

We investigated the morphological features of a freeze-concentrated solution (FCS) formed in frozen aqueous dimethyl sulfoxide (DMSO) solutions for cell cryopreservation. Specifically, we investigated the effects of cooling rates and initial DMSO concentrations on FCS morphology. At a cooling rate of 1 ℃/min, relatively large FCS channels formed owing to the crystallization of the extracellular ice crystals, accommodating cells effectively. Conversely, rapid cooling rates resulted in fine ice crystals, leading to the formation of narrower FCS channels. Slow cooling promoted cell accommodation, attributed to ice crystal reorientation and larger FCS sizes, underscoring the impact of FCS width profiles on cell accumulation during freezing. The recovery of C2C12 myoblasts was also studied by altering the freezing rate. The cell recovery rate of C2C12 myoblasts demonstrated that slow cooling improved recovery, whereas rapid cooling decreased it. Medium cooling rates showed greater variability in recovery, suggesting reduced consistency in cryopreservation outcomes. While numerous factors determine cell viability during freezing processes, our findings highlight FCS morphology as a critical determinant in designing effective cryopreservation protocols.

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初始冷却速率对冷冻水-二甲基亚砜介质中细胞保存的影响：形态学研究。

我们研究了冷冻浓缩溶液（FCS）在冷冻二甲基亚砜（DMSO）溶液中形成的细胞冷冻保存的形态学特征。具体来说，我们研究了冷却速率和初始DMSO浓度对FCS形态的影响。在1℃/min的冷却速率下，由于细胞外冰晶的结晶，形成了较大的FCS通道，有效地容纳了细胞。相反，快速的冷却速率导致细冰晶的形成，导致更窄的FCS通道的形成。缓慢冷却促进了细胞调节，这归因于冰晶重新定向和更大的FCS尺寸，强调了FCS宽度分布对冻结期间细胞积累的影响。通过改变冷冻速率，研究了C2C12成肌细胞的恢复情况。C2C12成肌细胞的细胞恢复速率表明，缓慢冷却提高了细胞恢复速率，而快速冷却降低了细胞恢复速率。中等冷却速率显示出更大的恢复变异性，表明低温保存结果的一致性降低。虽然在冷冻过程中有许多因素决定细胞活力，但我们的研究结果强调FCS形态是设计有效冷冻保存方案的关键决定因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.